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ProteomicsAnalysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT)
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614) 
2016-03-17 | MSV000079594 | MassIVE
UnknownPeptide labeling with isobaric tags yields higher identification rates using iTRAQ 4-plex compared to TMT 6-plex and iTRAQ 8-plex on LTQ Orbitrap.
Not available
S-EPMC3093924 | biostudies-literature
ProteomicsProteomics analysis of different stages during mouse hepatic ischemia-reperfusion injury
6-plex TMT-based quantitative proteomics was used to analysis the proteomic profile in different stages of mouse hepatic ischemia-reperfusion injury.
ORGANISM(S): Mus musculus (Mouse) 
2017-11-23 | PXD007827 | Pride
Mouse 6-plex tmt Liver 
ProteomicsTMT-based proteomics analysis of mouse germ cells
The proteome changes were quantified in RibosomeRPL39L-/- spermatocytes and elongated spermatids using TMT 6-plex, and in spermatogonia, spermatocytes, round spermatids and elongated spermatids using TMT 10-plex by LC-MS/MS.
ORGANISM(S): Mus musculus (Mouse) 
2022-10-05 | PXD020874 | Pride
Germ cells Tmt Mouse 
ProteomicsControl vs Elp3-deficient mouse myeloid progenitors 6-plex TMT LC-MSMS
6-plex TMT LC-MSMS quantification of proteins in myeloid progenitors (Lineage- Sca1- Kit+ cells) isolated from the bone marrow of 8-12 week old male and female control and Elp3-deficient (Elp3fl/fl vav-iCreT/+) mice. Biological triplicates of each genotype were analyzed, each consisting of 1.10e6 ce...
ORGANISM(S): Mus musculus (Mouse) 
2021-01-26 | PXD010486 | Pride
Elongator Myeloid progenitor Elp3 Trna Hematopoiesis 
ProteomicsTMT-based proteomics analysis of proteome changes in RNF114-/- mouse oocytes
The proteome changes were quantified in RNF114-/- mouse oocyte using TMT 6-plex by LC-MS/MS.
ORGANISM(S): Mus musculus (Mouse) 
2021-06-16 | PXD023458 | Pride
Proteomics Tmt Mouse Oocyte 
ProteomicsK - TMT-6 plex labelled plasma ratio's 1:1:3:3:9:9
TMT-6 plex labelled plasma ratio's 1:1:3:3:9:9
ORGANISM(S): Homo sapiens (Human) 
2010-05-26 | PRD000247 | Pride
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsTMT-based phosphoproteomics and proteomics analysis in Stk33-/- mouse testis
The phosphoproteome and proteome changes were quantified in Stk33-/- mouse testis using TMT 6-plex by LC-MS/MS.
ORGANISM(S): Mus musculus (Mouse) 
2023-07-20 | PXD036903 | Pride
Phosphoproteomics Proteomics Tmt Testis Stk33 
Proteomicsn Efficient, Amine-Specific, and Cost-Effective Method for TMT 6/11-plex Labeling Improves the Proteome Coverage, Quantitative Accuracy and Precision
Tandem mass tags (TMT) are among the most popular proteomics quantification techniques due to its ability to simulta-neously quantify multiple samples in a single experiment. The tags can be easily added onto the primary amine groups of peptides and proteins through chemical reactions. Although TMT ...
ORGANISM(S): Homo Sapiens 
2023-11-17 | PXD047052 |
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