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ProteomicsOptimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing
In large-scale quantitative mass spectrometry (MS)-based phosphoproteomics, isobaric labeling with tandem mass tags (TMTs) coupled with offline high-pH reversed-phase peptide chromatographic fractionation maximizes depth of coverage. To investigate to what extent limited sample amounts affects sensi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-28 | PXD031277 | Pride
Tandem mass tags Phosphoproteomics Optimization High-ph fractionation Isobaric labeling 
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
2021-07-23 | PXD025536 | JPOST Repository
ProteomicsTMT-based proteomics and phosphoproteomics analysis of proteome changes in seminal plasma extracellular vesicles of azoospermia
The proteome and phosphoproteomics changes were quantified in seminal plasma extracellular vesicles among healthy individuals with normal sperm, nonobstructive azoospermia and obstructive azoospermia using TMT 10-plex by LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022843 | Pride
Tmt  azoospermia  extracellular vesicles  seminal plasma  proteomics 
ProteomicsQuantitative phosphoproteomics study for assessing rodent thyroid toxicity
Here, we aimed to apply a TMT based quantitative phosphoproteomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm f...
ORGANISM(S): Rattus norvegicus (Rat) 
2024-10-25 | PXD030254 | Pride
Phosphoproteomics  liver  tmt Thyroid toxicity  ptu Phenytoin 
ProteomicsEvaluation of differential peptide loading on TMT-based proteomic and phosphoproteomic data quality
Investigating the impacts on MS data quantitation reproducibility when loading differential amounts of peptides, ranging from 20 to 400 ug, across channels of TMT 11 multiplexes. PTRC_Exp9 files represent global proteome and phosphoproteome data generated from PBMCs isolated from AML patients, acqui...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-05 | MSV000086417 | MassIVE
ProteomicsGlobal ion suppression limits the potential of mass spectrometry based phosphoproteomics
Mass spectrometry-based proteomics has become the method of choice to pinpoint and monitor thousands of post-translational modifications, predominately phosphorylation sites, in cellular signaling studies. Critical for achieving this analytical depth is the enrichment for phosphorylated peptides pri...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-02 | PXD007528 | Pride
Macrophages Tmt Phosphoproteomics Infection Ion supression Lc-ms/ms 
ProteomicsQuantitative phosphoproteomics explain cryopreservation-induced reductions in ram sperm motility
Our objective was to use quantitative phosphoproteomics for systematic comparative analyses of fresh versus frozen-thawed sperm to identify factors causing cryo-injury.
ORGANISM(S): Ovis Aries 
2023-12-28 | PXD048166 |
UnknownTemporal Quantitative Phosphoproteomics Profiling of Interleukin-33 Signaling Network Reveals Unique Modulators of Monocyte Activation.
Not available
S-EPMC8749991 | biostudies-literature
ProteomicsQuantitative phosphoproteomic analysis reveals system-wide signaling pathways regulated by site-specific phosphorylation on Keratin-8 in skin squamous cell carcinoma derived cell-line
The epithelial specific keratin pair, Keratin 8/18 (K8/18), has been reported to be aberrantly expressed in squamous cell carcinoma (SCC) which is also correlated with increased invasiveness and poor prognosis. A Majority of Keratin 8 (K8) functions are governed by its phosphorylation at Serine73 (...
ORGANISM(S): Homo sapiens (Human) 
2018-07-05 | PXD005301 | Pride
Keratin 8 phosphorylation Tmt based quantitative phosphoproteomics Human epidermoid carcinoma cell-line 
ProteomicsQuantitative tyrosine phosphoproteomics of wild type and PTPRK KO tumour xenografts
TMT-based quantitation of tyrosine phosphoproteomes of wild type and PTPRK KO tumour xenografts to determine additional mechanisms of tumour suppression by receptor tyrosine phosphatase PTPRK
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD050617 | Pride
Tmt Phosphatase Ptprk Tyrosine phosphoproteomics Tumour xenografts 
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