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UnknownDeep Coverage of Global Protein Expression and Phosphorylation in Breast Tumor Cell Lines Using TMT 10-plex Isobaric Labeling.
Not available
S-EPMC5336479 | biostudies-literature
ProteomicsProteomics analysis of human islets treated with IL-1b and IFN-g
Human islets were treated with IL-1b and IFN-g for 24h, digested with trypsin, multiplexed with TMT-10, fractionated by high pH reverse-phase chromatography and analyzed by tandem LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2019-10-25 | PXD009131 | Pride
Proteomics Tmt-10 Cytokines Human islets Type 1 diabetes Cell death 
ProteomicsProteomics analysis of EndoC-betaH1 cells treated with IL-1b and IFN-g
EndoC-betaH1 cells were treated with IL-1b and IFN-g for 48h, digested with trypsin, multiplexed with TMT-10, fractionated by high pH reverse-phase chromatography and analyzed by tandem LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2019-09-26 | PXD011902 | Pride
Beta cells Proteomics Tmt-10 Cytokines Type 1 diabetes Cell death 
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
2021-07-23 | PXD025536 | JPOST Repository
ProteomicsThe baseline vitreous proteome in rhegmatogenous retinal detachment: a case–control study of proliferative vitreoretinopathy
Quantitative proteomic analysis of undiluted human vitreous biopsies collected at the time of primary surgical repair of rhegmatogenous retinal detachment (RRD), comparing patients who later developed proliferative vitreoretinopathy (PVR; n=8) with matched RRD controls who did not (n=8). Vitreous sa...
ORGANISM(S): Homo sapiens (Human) 
2026-05-05 | PXD077831 | Pride
Pvr Tmt-10-plex Proteomics Vitreous Ocular Retinal detachment Biomarker Proliferative vitreoretinopathy 
ProteomicsTMT 10-plex based quantitative proteomic analysis of drug resistant breast cancer cell lines
Breast cancer accounts for roughly 30% of all cancers in women worldwide, has a 15% death rate, and incidence rates are increasing at a rate of about 0.5% per year. Breast cancer comprises a heterogeneous group of tumor subtypes, whether defined by the histopathology of the primary tumor, the expres...
ORGANISM(S): Homo sapiens (Human) 
2022-04-04 | PXD030881 | Pride
Human Tmt Breast cancer Drug resistance 
ProteomicsYTHDF1 links hypoxia adaptation and non-small cell lung cancer progression
10plex TMT labelling quantification expriments were performed to access the knockdown effect of YTHDF1 in cells.3 shGFPs control samples, 3 YTHDF1 shRNA-2 knockdown samples , 3 YTHDF1 shRNA-3 knockdown samples and 1 mixture sample were labelled by TMT 10-plex reagents.
ORGANISM(S): Homo sapiens (Human) 
2019-09-27 | PXD015182 | Pride
Lc-ms/ms tmt10plex 
Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsBenefits of FAIMS to improve the proteome coverage of hard-to-analyze TMT 10-plex FFPE tissue and plasma-derived exosomes samples
The analysis of complex protein mixtures, such as FFPE tissue or plasma exosomes samples, is a challenge nowadays due to the low proteome coverage achieved by mass spectrometers nowadays. We have here investigated the benefits of FAIMS technology coupled to the Orbitrap Exploris 480 mass spectromete...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD042597 | Pride
Orbitrap exploris 480 Mass spectrometry Proteomics Faims Ffpe tissue Exosomes 
ProteomicsBenefits of FAIMS to improve the proteome coverage of hard-to-analyze TMT 10-plex FFPE tissue and plasma-derived exosomes samples
The analysis of complex protein mixtures, such as FFPE tissue or plasma exosomes samples, is a challenge nowadays due to the low proteome coverage achieved by mass spectrometers nowadays. We have here investigated the benefits of FAIMS technology coupled to the Orbitrap Exploris 480 mass spectromete...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD042596 | Pride
Orbitrap exploris 480 Mass spectrometry Proteomics Faims Ffpe tissue Exosomes 
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