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ProteomicsMultiple sclerosis resaerch: EAE and cuprizone mouse brain TMT and label-free proteomics
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system, and in this study we used quantitative proteomics to compare the brain proteomes of two MS mouse models, the immune-mediated experimental autoimmune encephalomyelitis (acute and recovery phase) and...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-16 | PXD002318 | Pride
Orbitrap velos Mus musculus Brain Tmt Label-free 
ProteomicsA systematic comparison of label-free, SILAC and TMT techniques to study early adaption towards inhibition of EGFR signaling in the colorectal cancer cell line Difi
The epithelial growth factor receptor (EGFR) signalling network plays an essential role inproliferation and survival of colorectal cancer cells. For this reason, EGFR is targeted using therapeutic antibodies blocking EGFR signalling (e.g. cetuximab). However, anti-EGFR therapy is rarely curative as ...
ORGANISM(S): Homo sapiens (Human) 
2019-12-11 | PXD014565 | Pride
Phosphoproteomics Tmt Anti-egfr therapy Colorectal cancer Silac Mapk Label-free Erbb3 Adaptive mechanism Cetuximab 
ProteomicsIsolation, enrichment and identification of p62 condensates components by GFP-Trap Agarose Beads pulldown followed by Label Free and Tandem Mass Tag (TMT) mass spectrometry analysis from Hap1 cells
Selective autophagy contributes to the removal of harmful proteins from the cytoplasm. This cargo material is selected by cargo receptors such as p62/SQSTM1 and finally degraded. However, the molecular composition of the p62 condensates and therefore the nature of the cargo delivered by them is inco...
ORGANISM(S): Homo sapiens (Human) 
2023-11-22 | PXD039661 | Pride
Autophagy  cargo receptor  sqstm1  translation 
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsRatio accuracy evaluation and correction using tandem mass tags
The multiplexing capabilities of isobaric mass tag based protein quantification, such as Tandem Mass Tags (TMT) or Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) have dramatically increased the scope of Mass Spectrometry (MS) based proteomics studies. Not only does the technology allow ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-27 | MSV000082855 | MassIVE
ProteomicsTesting TMT-SPS-MS3 and label free approach in recalcitrant Mago (Mangifera indica L.) peel tissues
Significant progress has been observed in recent years in proteomics tools. However, minimal application of the newest proteomic tools has been massively applied in plant recalcitrant tissues in non-model fruits. This constant occurrence constrains the progress in understanding essential molecular p...
ORGANISM(S): Mangifera indica (Mango) 
2024-01-29 | PXD040720 | Pride
Protein extraction Proteomics Postharvest shelf life Mango peel 
ProteomicsProteome-wide evaluation of two common protein quantification methods
Proteomics experiments commonly aim to estimate and detect differential abundance across all expressed proteins. Within this experimental design, some of the most challenging measurements are small fold changes for lower abundance proteins. While bottom-up proteomics methods are approaching comprehe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-02-15 | MSV000083453 | MassIVE
UnknownComparative Proteome Profiling of Saliva Between Estrus and Non-Estrus Stages by Employing Label-Free Quantitation (LFQ) and Tandem Mass Tag (TMT)-LC-MS/MS Analysis: An Approach for Estrus Biomarker Identification in Bubalus bubalis.
Not available
S-EPMC9217162 | biostudies-literature
ProteomicsPeli1 knock-out and wild type mouse brain proteomes during the disease course of experimental autoimmune encephalomyelitis
The ubiquitin ligase Peli1 has previously been suggested as a potential treatment target in multiple sclerosis since the knock-out induced less activated microglia and less inflammation in the CNS of experimental autoimmune encephalomyelitis in mice. In the present study the brain proteomes of Peli1...
ORGANISM(S): Mus musculus (Mouse) 
2016-09-27 | PXD003710 | Pride
Tmt Label-free Eae Cns Peli1 Multiple sclerosis Quantitative proteomics 
ProteomicsSpatial and temporal proteomic of HCMV-infected cells
Project for the study of spatial and temporal alterations in organelle proteins during HCMV infection. Two datasets are included, one for label-free quantification and one for TMT quantification. Samples were collected at 5 time points of infection (24, 48, 72, 96, and 120 hours) and subject to orga...
ORGANISM(S): Homo sapiens (Human) Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) 
2016-09-27 | PXD003925 | Pride
Hcmv spatial organelle temporal cytomegalovirus fibroblasts 
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