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ProteomicsTMT-labeled quantitative proteomes of nine tick bodies
The analysis workflow of this project mainly comprises two stages: mass spectrometry experiment and data analysis. The analysis process of the mass spectrometry experiment mainly involves steps such as protein extraction, enzymatic digestion of peptides, TMT (Tandem Mass Tag) labeling, chromatograph...
ORGANISM(S): Hyalomma anatolicum anatolicum 
2026-03-19 | PXD058399 | Pride
Tick Tmt-labeled quantitative proteomes 
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
PXD025536 | JPOST Repository
ProteomicsProteome-wide multipoint internal calibration curves for mass spectrometry-based quantitative proteomics
Mass spectrometry (MS)-based proteomics is known for its high accuracy in quantifying peptides and proteins using various calibration strategies, including internal and external calibration curves. While external multi-point calibration curves are created from serial dilutions, they often fail to ac...
ORGANISM(S): Homo sapiens (Human) 
2025-02-24 | PXD059628 | Pride
Quantitative Ovarian cancer Multipoint Tmt Calibration curves 
UnknownSurveying the Proteome-Wide Landscape of Mitoxantrone and Examining Drug Sensitivity in BRCA1-Deficient Ovarian Cancer Using Quantitative Proteomics.
Not available
S-EPMC12641731 | biostudies-literature
UnknownQuantitative urinary proteome analysis reveals potential biomarkers for disease activity of Behcet's disease uveitis.
Not available
S-EPMC11232131 | biostudies-literature
Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsMass spectrometry–based proteomic profiling of pleural tissue
This dataset contains mass spectrometry-based proteomic data generated from pleural tissuesamples.Proteins were extracted, digested, and labeled using TMT isobaric labeling for quantitative analysis. Samples were analyzed using LC–MS/MS to profile the global proteome.The dataset includes raw mass sp...
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD073793 | Pride
Mass spectrometry  lc–ms/ms  pleural tissue 
Unknown29-Plex tandem mass tag mass spectrometry enabling accurate quantification by interference correction.
Not available
S-EPMC9588555 | biostudies-literature
ProteomicsQuantitative Proteomics Analysis with TMT Markers
Using TMT-labeled quantitative protein technique, we quantitatively analyzed the proteomes of three different resistant Bambusa pervariabilis × Dendrocalamopisis grandis under four different treatments: pathogenic fungi, pathogenic toxin, pathogenic fungi plus toxin and sterile water, and screened o...
ORGANISM(S): Bambusa Pervariabilis Bambusa Grandis 
PXD014363 |
ProteomicsHigh-flow chromatography for reproducible and high-throughput quantification of proteomes
This study evaluates liquid-chromatography operated at 50 ul/min using 1 mm diameter columns coupled online to tandem mass spectrometry (LC-MS/MS) for proteome analysis. Data from >1,000 human cell line, tissue, body fluid, affinity- and phospho-proteomes demonstrate excellent chromatographic (<0.3 ...
ORGANISM(S): Homo sapiens (Human) 
2019-12-09 | PXD015087 | Pride
High-flow lc Chromatography Proteomics Body fluids Kinobeads 
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