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TranscriptomicsLigand-Dependent Enhancer Activation Regulated by Topoisomerase-I Activity
The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve torsional stress f...
ORGANISM(S): Homo sapiens 
2015-01-22 | E-GEOD-63202 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of novel splice variants induced by topoisomerase I poisoning shows preferential occurrence in genes encoding splicing factors
Topoisomerase I (Top1) relaxes both positive and negative supercoilings by producing transient Top1 cleavage complexes (Top1cc). Several studies have suggested the implication of Top1 in splicing. Here, we tested the implication of Top1cc in splicing at the global genome level in human carcinoma cel...
ORGANISM(S): Homo sapiens 
2011-07-21 | E-GEOD-23677 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human hepatocytes treated with doxorubicin
A variety of important anticancer drugs kill cells by increasing cellular levels of topoisomerase II-DNA cleavage complex. The anthracycline anticancer drug doxorubicin forms a stable ternary complex with DNA and topoisomerase IIa, thereby inhibiting the normal function of the enzyme. In this study ...
ORGANISM(S): Homo sapiens 
2008-10-10 | E-GEOD-11940 | biostudies-arrayexpress
ProteomicsTEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts
Eukaryotic topoisomerase 1 (TOP1) regulates DNA topology to ensure efficient DNA replication and transcription. TOP1 is also a major driver of endogenous genome instability, particularly when its catalytic intermediate - a covalent TOP1-DNA adduct known as a TOP1 cleavage complex (TOP1cc) - is stabi...
ORGANISM(S): Homo sapiens (Human) 
2020-03-13 | PXD017239 | Pride
Protein-dna adducts Vcp/p97 Top1 Tex264 Dna repair Genome stability Sprtn Dna replication 
ProteomicsTopoisomerase I Poisoning by Electrophiles Derived from Oxidative Stress
The cancer target enyzme topoisomerase 1 transiently cleaves one strand of chromosomal DNA to relax accumulated strain that can prevent transcription, replication or chromatin assembly. The topoisomerase 1 poison camptothecin and its synthetic analogs are widely used chemotherapeutics that act by tr...
ORGANISM(S): Homo sapiens (Human) 
2021-03-24 | PXD018549 | Pride
Oxidative stress Chemotherapy Q-exactive Topoisomerases Enzymology Proteomics 
TranscriptomicsTopoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms
Topoisomerase 1 (TOP1) inhibitors, including camptothecin and topotecan, covalently trap TOP1 on DNA, creating cleavage complexes (cc’s) that must be resolved before gene transcription and DNA replication can proceed. We previously found that topotecan reduces the expression of long (>100 kb) genes ...
ORGANISM(S): Mus musculus 
2016-05-27 | GSE79951 | GEO
UnknownTopoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms.
Not available
S-EPMC4883752 | biostudies-literature
UnknownMechanisms controlling replication fork stalling and collapse at topoisomerase 1 cleavage complexes.
Not available
S-EPMC7617106 | biostudies-literature
GenomicsNEDDylated-Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors (ChIP-Seq)
NEDDylated-Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors (ChIP-Seq)
PRJNA875475 | ENA
ProteomicsTopoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence
SILAC-MS analysis to determine the mechanism by which HMGB2 regulates cGAS localization into CCF during senescence.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-10 | MSV000084789 | MassIVE
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