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ProteomicsCytoplasmic complex comprising TAF2, TAF8, TAF10 provides evidence for nuclear holo-TFIID assembly from preformed submodules
General transcription factor TFIID is a cornerstone of RNA polymerase II transcription initiation in eukaryotic nuclei. Human TFIID is a megadalton-sized multiprotein complex composed of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). The cellular mechanism of TFIID assembly is ...
ORGANISM(S): Homo sapiens (Human) 
2016-06-15 | PXD001427 | Pride
Assembly Tfiid Tafstructure Mudpit analysis 
ProteomicsHuman KAT2A/B-acetylome MudPIT
Comprehensive identification of acetylated proteins targeted by the lysine acetyltransferases KAT2A (GCN5) and KAT2B (PCAF), in human cells. KAT2A/B-dependent acetylated proteins were identified by comparing acetylated proteins in whole cell extract of control cells and cells in which KAT2A and KAT2...
ORGANISM(S): Homo sapiens (Human) 
2016-12-12 | PXD004669 | Pride
Acetylation Non-histone targets Kat2a(gcn5) Centrosome duplication Kat2b (pcaf) Plk4 
ProteomicsTAF8 MUTATION CAUSES INTELLECUAL DISABILITY, TFIID DISASSEMBLY WITH NO EFFECT ON RNA POLYMERASE II TRANSCRIPTION
The human general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). In eukaryotic cells, TFIID nucleates RNA Polymerase II (Pol II) preinitiation complex formation on gene promoters and thus, is crucial for Pol II transcription. Germline k...
ORGANISM(S): Homo sapiens (Human) 
2018-04-16 | PXD008084 | Pride
Tata binding protein (tbp) Chromatin immunoprecipitation (chip) Frame shift Intellectual disability Rna polymerase ii (pol ii) Patient Tfiid Tbp-associated factor (taf) Splice site mutation 
GenomicsRNA polymerase II pausing downstream of core histone genes is different from genes producing polyadenylated transcripts
Recent genome-wide chromatin immunoprecipitation coupled high throughput sequencing (ChIP-seq) analyses performed in various eukaryotic organisms, analysed RNA Polymerase II (Pol II) pausing around the transcription start sites of genes. In this study we have further investigated genome-wide binding...
ORGANISM(S): Homo sapiens 
2012-06-15 | E-GEOD-34001 | biostudies-arrayexpress
TranscriptomicsTAF15 protein regulates CDKN1A through miRNAs
TAF15 (formerly TAFII68) is a member of the TET family of RNA and DNA binding proteins whose genes are frequently translocated in sarcomas. Consistent with a functional role in cell viability, TAF15 depletion had a growth-inhibitory effect and increased apoptosis. Interestingly, one of the genes aff...
ORGANISM(S): Homo sapiens 
2012-12-21 | E-GEOD-34470 | biostudies-arrayexpress
ProteomicsArchitecture of TAF11/TAF13/TBP complex suggests novel regulatory state of human general transcription factor TFIID
General transcription factor TFIID is a key component of RNA polymerase II transcription initiation in eukaryotic nuclei. Human TFIID is a megadalton-sized multiprotein complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core promoter DNA, recognizing the...
ORGANISM(S): Homo sapiens (Human) 
2017-11-15 | PXD005676 | Pride
Tfiid  taf11  taf13  dna binding  tata-binding protein  histone fold 
ProteomicsSUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells
Co-activator complexes regulate chromatin accessibility and transcription. SAGA (Spt-Ada-Gcn5 Acetyltransferase) is an evolutionary conserved multisubunit co-activator complex with modular organization. The core module of SAGA constitutes the structural heart, composed of a histone octamer like stru...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-07-26 | PXD026991 | Pride
Elisabeth scheer Didier devys Damien plassard Elisabeth lata bastian morlet And lászló tora Veronique fischer Stéphane d. vincent 
GenomicsATAC and Mediator coactivators form a stable complex and regulates a set of non-coding RNA genes
The ATAC histone acetyl-transferase (HAT) and the Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here we report the identification of a new stable molecular assembly formed between the ATAC and the Mediator complex in mouse embr...
ORGANISM(S): Mus musculus 
2010-06-24 | E-GEOD-21717 | biostudies-arrayexpress
GenomicsSAGA and ATAC histone acetyl transferase complexes regulate distinct sets of genes and ATAC defines a class of p300-independent enhancers
Metazoan SAGA and ATAC are distinct multi-subunits complexes that share the same catalytic HAT subunit (GCN5 or PCAF). Here we show that these human HAT complexes are targeted to different genomic loci representing functionally distinct regulatory elements both at broadly expressed and tissue specif...
ORGANISM(S): Homo sapiens 
2011-11-05 | E-GEOD-31052 | biostudies-arrayexpress
TranscriptomicsTAF10 interacts with the GATA1 transcription factor and controls mouse erythropoiesis
We have ablated TAF10 in the erythroid compartment only by crossing the TAF10lox mice with the EpoR-Cre mice and we have studied the development of the erythroid cells in vivo. TAF10 ablation led to embryonic death at E13.5 while at E12.5 there was a clear developmental defect which was reflected in...
ORGANISM(S): Mus musculus 
2015-04-21 | E-GEOD-68083 | biostudies-arrayexpress
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