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ProteomicsIdentification of protein N-termini in Cyanophora paradoxa cyanelles: Transit peptide composition and sequence determinants for precursor maturation
Glaucophytes, rhodophyta and chloroplastida represent the three evolutionary lineages that diverged after primary endosymbiosis. Comparative analyses between members of these three lineages are a rich source of information on ancestral plastid features. We analyzed the composition and the cleavage s...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-14 | PXD002187 | Pride
Cyanophera paradoxa Ms Chloroplast transit peptide Tails 
UnknownIdentification of protein N-termini in Cyanophora paradoxa cyanelles: transit peptide composition and sequence determinants for precursor maturation.
Not available
S-EPMC4510345 | biostudies-literature
ProteomicsHeterologous Expression of MBP-AtABC1K6-His in E. coli
Heterologous E. coli expression of AtABC1K6 tagged with maltose binding protein (MBP) and 6xHis tags revealed a spontaneous cleavage event of the recombinant protein during purification. Resulting bands were resolved on SDS-PAGE and excised for MS/MS identification. Mass spec-based identification of...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD030384 | Pride
Coq8 Kinase Spontaneous cleavage Ubib Abc1 
TranscriptomicsTARBP2 HITS-CLIP
In order to identify TARBP2 binding sites on endogenous RNA, we performed HITS-CLIP on a myc-tagged TARBP2 expressing cell-line (transient transfection) Cells were transfected with tagged TARBP2 vector (Origene) and 48-hr post-transfection, they were subjected to the HITS-CLIP procedure (Licatalosi ...
ORGANISM(S): Homo sapiens 
2014-07-24 | E-GEOD-49648 | biostudies-arrayexpress
UnknownHeterologous expression of Bixa orellana cleavage dioxygenase 4-3 drives crocin but not bixin biosynthesis.
Not available
S-EPMC8896647 | biostudies-literature
ProteomicsMaturation, site of developmental origin and haematopoietic state dictate murine neutrophil function
Maturation, age, tissue localization and functional capacity all drive neutrophil heterogeneity. In mouse models of cancer, we found that Ly6GInt neutrophils were abundant, and their frequency correlated with metastatic potential. Only Ly6G surface expression was found to accurately identify neutrop...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-05 | PXD026708 | Pride
Maturation Neutrophil Flow cytometry Tmt proteomic 
UnknownA chloroplast processing enzyme functions as the general stromal processing peptidase.
Not available
S-EPMC22651 | biostudies-literature
TranscriptomicsRegulatory consequences of TARBP2 over-expression in MDA-231 cell-line
Transient transfection of TARBP2 expression vectors (along with GFP controls) followed by transcriptomic profiling revealed that over-expression of TARBP2 resulted in a significant enrichment of sRSE-carrying transcripts among the down-regulated transcripts. Transient transfection of CMV-driven TARB...
ORGANISM(S): Homo sapiens 
2014-07-24 | E-GEOD-56948 | biostudies-arrayexpress
UnknownAssembly of Human Papillomavirus 16 L1 Protein in Nicotiana benthamiana Chloroplasts into Highly Immunogenic Virus-Like Particles.
Not available
S-EPMC10078042 | biostudies-literature
ProteomicsProteomic profiling of RNA binding proteins in mouse spermatogonial stem cell
RNA-binding proteins (RBPs) control the fate of RNAs. Tissue regeneration and homeostasis depend on tissue-specific stem cells, but the scope of RBP involvementin these processes remains largely unknown. Here, we identified the RBP repertoire of the mouse undifferentiated spermatogonial population t...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-19 | PXD017377 | Pride
Mouse Shotgun proteomics Mrna interactome capture Rna-binding proteins Spermatogonial stem cell 
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