Transcriptional profiling of hESCs in chemically-defined culture media compared to hESCs differentiated for 36h in the additional presence of FGF, LY294002 and either BMP or ActivinA.
Comparison of H9 human embryonic stem cells, grown in chemically defined and animal-product free conditions, with their differentiated progeny - mesendodermal progenitors arisen as the result of changes made to chemically defined hESC growth conditions. Contributes to a larger work on the use of che...
Transcriptional profiling comparison of mouse embryonic stem cells versus their JAK mutated counterparts, cultured both in the presence and absence of LIF (leukemia inhibitory factor).
Transcriptional comparison of mouse embryonic stem cells, inner cell mass, epiblast and pluripotent cells derived from mouse epiblast under defined culture conditions.
Activin/Nodal signalling is necessary to maintain pluripotency of human Embryonic Stem Cells (hESCs) and to induce their differentiation towards endoderm. However, the mechanisms by which Activin/Nodal signalling achieves these opposite functions remain unclear. To unravel these mechanisms, we exami...
Understanding the molecular mechanisms controlling early cell fate decisions in mammals is a major objective towards the development of robust methods for the differentiation of human pluripotent stem cells into clinically relevant cell types. Here, we used human embryonic stem cells (hESCs) to stud...
Gene expression microarray analysis of hESCs treated with BMP4 in chemically-defined medium, with and without inhibitors of Activin and FGF signalling (which maintain pluripotency), to investigate the induction of extra-embryonic tissue (trophectoderm) differentiation by BMP signalling.
