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UnknownApplication of Strep-Tactin XT for affinity purification of Twin-Strep-tagged CB2, a G protein-coupled cannabinoid receptor.
Not available
S-EPMC5406253 | biostudies-literature
UnknownBroad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein.
Not available
S-EPMC3680311 | biostudies-literature
ProteomicsAPPLE-MS: A modular affinity purification-mass spectrometry method assisted by PafA- mediated proximity labeling
While affinity purification-mass spectrometry (AP-MS) has significantly advanced protein-protein interaction (PPI) studies, its limitations in detecting weak, transient, and membrane-associated interactions remain. To address these challenges, we introduced an innovative proteomic method termed Affi...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2025-10-27 | PXD063145 | Pride
Ap-ms Proximity labeling Protein-protein interaction Interactome 
ProteomicsSARS-CoV-2 nsp14 induces the m7G methylation and the sequestration of host RNAs in stress granules Twin-Strep Streptactin pull-down LC-MSMS
Viruses grab the host translation machinery to facilitate their replication by disturbing the host translation. SARS-CoV-2 nsp14 was known to inhibit host translation, yet the mechanistic details are still obscure. In this study, we report that nsp14 can elevate stress granules (SG) formation in a N...
ORGANISM(S): Homo sapiens (Human) 
2026-02-06 | PXD060757 | Pride
Lc-msms Nsp14 Twin-strep Stress granules 
ProteomicsSTIC2 selectively binds ribosome-nascent chain complexes in the cotranslational sorting of Arabidopsis thylakoid proteins
Chloroplast-encoded multi-span thylakoid membrane proteins are crucial for photosynthetic complexes, yet the coordination of their biogenesis remains poorly understood. To identify factors that specifically support the cotranslational biogenesis of the reaction center protein D1 of photosystem (PS) ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-08-28 | PXD042896 | Pride
Thylakoid membrane Photosystem ii Arabidopsis Cotranslational targeting Stic2 
GenomicsChIP-seq of H2A.Z and its histone chaperones during the cell cycle
The aim of this study was to uncover cell cycle dependent chromatin binding of H2A.Z and its histone chaperones. U2OS cells were stably transfected with Twin-Strep-tagged H2A.Z, ANP32e, or YL1 constructs, and WT cells were used as a negative/background control. U2OS cells were synchronised in G1 or ...
ORGANISM(S): Homo sapiens 
2023-06-29 | E-MTAB-11617 | biostudies-arrayexpress
UnknownSpecific Protein Quantification by Radioimmuno-Dot-Blot Assay for Complex Mixture Samples Utilizing Strep-Tag and Tritium-Labeled Strep-Tactin.
Not available
S-EPMC11755393 | biostudies-literature
ProteomicsDysferlin and its role in cardiac disease
To explore the proteomic interactome of Dysferlin in the heart, we used recombinant expression of N-terminally Twin-Strep II-tagged full-length human Dysferlin to establish an in vitro binding assay. Isolated mouse heart membrane fractions were incubated with Dysferlin-coupled beads under four diffe...
ORGANISM(S): Mus musculus (Mouse) 
2024-07-13 | PXD045738 | Pride
Mouse heart tissue Dysferlin Label-free quantification Data-independent acquisition Interactome 
ProteomicsSystematic affinity purification mass spectrometry (AP-MS) reveals LCMV interacted human proteins
To systematically map the arenavirus‒host protein interaction, we employed a systematic affinity purification mass spectrometry (AP-MS) approach to define the human proteins that physically interact with LCMV proteins. The four LCMV proteins (NP, LP, GPC, Z) and control protein GFP were tandemly clo...
ORGANISM(S): Homo Sapiens 
2023-07-28 | PXD044162 |
ProteomicsIdentification of substrates of LarA-activated Lon protease and Identification of Lon protease interactome
With this analysis we aim to identify proteins that are substrates of LarA-activated Lon protease in Caulobacter crescentus. We will compare samples of wild type cells either harboring an empty vector (vector control, VC) or overexpressing 3xFLAG-tagged LarA (F-LarA). Based on our previous data we h...
ORGANISM(S): Caulobacter crescentus (strain NA1000 / CB15N) 
2023-10-25 | PXD036514 | Pride
Caulobacter crescentus Lon activity regulator Lara Lon protease 
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