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TranscriptomicsIdentification of cell cycle-regulated genes periodically expressed in U2OS cells and their regulation by FOXM1 and E2F transcription factors
We identified the cell cycle-regulated mRNA transcripts genome-wide in the osteosarcoma derived U2OS cell line. This resulted in 2,140 transcripts mapping to 1,871 unique cell cycle-regulated genes that show periodic oscillations across multiple synchronous cell cycles. We identified genomic loci ...
ORGANISM(S): Homo sapiens 
2013-10-11 | E-GEOD-50988 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human U2OS cells expressing estrogen receptor alpha and beta heterodimers and treated with 17beta-estradiol or 4OH-tamoxifen
U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-2292 | biostudies-arrayexpress
TranscriptomicsHigh temporal resolution profiling of U2OS fibroblasts
High-temporal resolution profiling was performed on U2OS fibroblasts to detect rhythmic transcripts Keywords: time course Samples were collected every hour for 48 hours from dexamethasone-synchronized U2OS cells. Cells synchronized with dexamethasone for 24 hours, and harvested from the 24th to 71...
ORGANISM(S): Homo sapiens 
2008-12-14 | E-GEOD-13949 | biostudies-arrayexpress
GenomicsWhole-genome CRISPR screen of etoposide-treated U2OS and U2OS p53KO cells, and RNAseq of U2OS cells incubated in media conditioned by etoposide-treated U2OS or U2OS p53KO cells.
Whole-genome CRISPR screen of etoposide-treated U2OS and U2OS p53KO cells, and RNAseq of U2OS cells incubated in media conditioned by etoposide-treated U2OS or U2OS p53KO cells.
PRJNA972777 | ENA
GenomicsHistone H3K18ac ChIP-seq in U2OS cells
Chromatin immuno-precipitation using anti-H3K18ac antibodies in U2OS cells.
ORGANISM(S): Homo sapiens 
2016-04-27 | E-MTAB-3695 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of H3K27ac, H3K4me1, H3K27me3 and H3K9me3 (ChIP-seq) in U2OS-GR and U2OS-AR cells
We performed ChIP-seq targeting the H3K27ac, H3K4me1, H3K27me3 and H3K9me3 in the U2OS-GR and U2OS-AR cell lines. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2OS-GR cells were treated with dexa...
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9617 | biostudies-arrayexpress
TranscriptomicsGlucocorticoid and androgen receptor profiling (ChIP-seq) in U2OS-GR and U2OS-AR cells upon hormone treatment
We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line and the androgen receptor (AR) in the U2OS-AR cell line. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2O...
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9616 | biostudies-arrayexpress
GenomicsHomo sapiens strain:U2OS
DDX17 CLIP-seq in U2OS cells with and without Rift Valley Fever Virus infection.
PRJNA244543 | ENA
ProteomicsInteractome of chromatin-associated RNA Polymerase II from U2OS cells
To determine the interactome of RNA Polymerase II (RNAPII), HA-tagged RPB3 was stably expressed in U2OS cells. The cells were harvested followed by the native stepwise isolation of chromatin-associated RNAPII complexes. HA-RPB3 was immunoprecipitated and interacting proteins analyzed by quantitative...
ORGANISM(S): Homo sapiens (Human) 
2024-12-20 | PXD054701 | Pride
Interactome U2os Rnapii 
ProteomicsProtein identification in 1000 U2OS cells
In this study, we aim to optimize sample processing methods using a conventional in-solution proteomic sample processing workflow. We then compared protein idetnfication numbers withour improved sample processing methods versus the original method, in 1000 U2OS human osteosarcoma cells.
ORGANISM(S): Homo sapiens (Human) 
2019-05-06 | PXD013702 | Pride
Sample recovery yield 1000 cells Lc-ms U2os Sam ple processing Osteosarcoma 
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