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ProteomicsMutant EGFR is a preferred SMURF2 substrate of ubiquitination: role in enhanced receptor stability and TKI sensitivity
We previously reported that differential protein degradation of TKI-sensitive [L858R, del(E746-A750)] and resistant (T790M) epidermal growth factor receptor (EGFR) mutants upon erlotinib treatment correlates with drug sensitivity. However, the molecular mechanism remains unclear. We also reported SM...
ORGANISM(S): Homo sapiens (Human) 
2020-07-29 | PXD018324 | Pride
Epidermal growth factor receptor (egfr)  smad ubiquitination regulatory factor 2 (smurf2)  ubiquitin conjugating enzyme h5 (ubch5)  protective ubiquitination  tyrosine kinase inhibitor (tki) resistance  lc-ms 
ProteomicsThe phosphatase DUSP22/JKAP inhibits UBR2-mediated K63-ubiquitination and activation of Lck in TCR signaling
DUSP22 (also named JKAP) is a dual-specificity phosphatase that inhibits T cell activation. Here we identified the E3 ubiquitin ligase UBR2 as an upstream activator of Lck during T-cell activation. JKAP dephosphorylated UBR2 at two residues, leading to ubiquitin-mediated UBR2 degradation. The SCF (S...
ORGANISM(S): Homo sapiens (Human) 
2024-01-04 | PXD043457 | Pride
Human jurkat t cells 
ProteomicsStUbEx PLUS in U2OS
StUbEx PLUS – a modified Stable tagged Ubiquitin Exchange system for Peptide Level purification and in-depth mapping of Ubiquitination Sites. Applying StUbEx PLUS to U2OS cells treated with proteasomal inhibitors MG132 or Bortezomib resulted in the identification of 41,582 sites mapping on 7,762 pro...
ORGANISM(S): Homo sapiens (Human) 
2022-03-01 | PXD006092 | Pride
Site specific ubiquitination  ubiquitination sites  n-terminal ubiquitination  proteasomal inhibition  mg132: bortezomib 
ProteomicsThe phosphatase DUSP22/JKAP inhibits UBR2-mediated K63-ubiquitination and activation of Lck in TCR signaling ( Sequential regulation of Lck K63-ubiquitination and autophosphorylation by UBR2 and DUSP22/JKAP in TCR signaling)
DUSP22 (also named JKAP) is a dual-specificity phosphatase that inhibits T cell activation. Here we identified the E3 ubiquitin ligase UBR2 as an upstream activator of Lck during T-cell activation. JKAP dephosphorylated UBR2 at two residues, leading to ubiquitin-mediated UBR2 degradation. The SCF (S...
ORGANISM(S): Homo sapiens (Human) 
2024-01-04 | PXD043454 | Pride
Hek293t mass 
ProteomicsUbiquitination sites on MIDN identified by mass spectrometry analysis
In E. coli, the pACYC-HA-Ub-E1 (UBA1)-UbcH5A-RNF126 and pET-22b-MIDN-His₆ plasmids were co-transformed. After IPTG induction to express all proteins required for ubiquitination, His-tagged MIDN was enriched with Ni-NTA resin and subjected to mass-spectrometric mapping of its ubiquitin-modified sites...
ORGANISM(S): Homo sapiens (Human) 
2026-06-01 | PXD069387 | Pride
Ubiquitination sites Midn 
UnknownMapping ubiquitination sites of S. cerevisiae Mcm10.
Not available
S-EPMC5421568 | biostudies-literature
ProteomicsMapping of TDP-43 ubiquitination and acetylation sites
TAR DNA binding protein of 43 kDa (TDP-43) forms aggregates in neurodegenerative diseases, particularly certain forms of frontotemporal dementia and amyotrophic lateral sclerosis. Pathological modifications of TDP-43 include proteolytic fragmentation, phosphorylation, and ubiquitinylation. In this s...
ORGANISM(S): Homo sapiens (Human) 
2022-04-01 | PXD030170 | Pride
Acetylation Ubiquitination Tdp-43 
ProteomicsHERC4 suppresses colorectal cancer progression by promoting STAT3 ubiquitination
Our study aims to identify the enzymes that interact with STAT3 and the specific sites where STAT3 undergoes ubiquitination modification.
ORGANISM(S): Homo Sapiens 
2026-03-24 | PXD076133 |
GenomicsPolySUMOylation restrains ubiquitination to protect fragile genomic loci during replication stress in fission yeast
PolySUMOylation restrains ubiquitination to protect fragile genomic loci during replication stress in fission yeast
PRJNA1458627 | ENA
ProteomicsDetection of STAT3 ubiquitination modification sites
Identify all the lysine sites of STAT3 that may be ubiquitinated,The search criteria were as follows: no enzyme was choosed as unspecific; two missed cleavage was allowed; carbamidomethylation (C) were set as the fixed modifications; the oxidation (M) and ubiquitination were set as the variable modi...
ORGANISM(S): Homo Sapiens 
PXD078167 |
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