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ProteomicsCRISPR/Cas9-mediated knockout and endogenous complex analysis of Cluap1/ IFT38
CRISPR/Cas9-mediated gene-editing allows manipulation of a gene of interest in its own chromosomal context. When applied to the analysis of protein interaction, and in contrast to an exogenous expression of a protein, these can be studied maintaining physiological stochiometries, topology and contex...
ORGANISM(S): Homo sapiens (Human) 
2018-04-04 | PXD008343 | Pride
Cilia Proteomics Crispr/cas9 Ift 
ProteomicsTTC30A and TTC30B redundancy protects IFT complex B integrity and its pivotal role in ciliogenesis
Intraflagellar transport (IFT) is a microtubule-based system that supports the assembly and maintenance of cilia, an essential organelle for development and maintenance of cellular integrity. Dysfunction of IFT was shown to lead to a wide spectrum of disease phenotypes, so called ciliopathies of var...
ORGANISM(S): Homo sapiens (Human) 
2023-12-28 | PXD033614 | Pride
Ift Cilia 
ProteomicsTissue-specific protein complex analysis with natively processed bait proteins
Protein-protein interaction analysis is an important tool to elucidate the function of proteins and protein complexes as well as their dynamic behavior. Despite the large toolset available to date the analysis of tissue specific complexes is still relying on the availability of specific antibodies s...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2023-12-28 | PXD018047 | Pride
Tissue-specific protein complex analysis Isoform-specific 
ProteomicsTissue- and isoform-specific protein complex analysis with natively processed bait proteins
Protein-protein interaction analysis is an important tool to elucidate the function of proteins and protein complexes as well as their dynamic behavior. To date, the analysis of tissue- or even cell- or compartment-specific protein interactions is still relying on the availability of specific antibo...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2023-12-28 | PXD020379 | Pride
Flag tag Tissue-specific complex 
ModelsTikidji-Hamburyan2018 - Rod phototransduction under strong illumination
Tikidji-Hamburyan2018 - Rod phototransduction under strong illumination This model is described in the article: Rods progressively escape saturation to drive visual responses in daylight conditions. Tikidji-Hamburyan A, Reinhard K, Storchi R, Dietter J, Seitter H, Davis KE, ...
MODEL1710030000 | BioModels
ProteomicsBovine tissue and blood contamination from bullets by LC-MS/MS
We have measured the proteomes of different major bovine organs in multiple replicate. Before the actual sample preparation, organs were penetrated with metal bullets experimentally. The biological material on these projectiles was then "on-surface" digested using trypsin and analyzed using LC-MS/MS...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606) 
2016-08-23 | MSV000080090 | MassIVE
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