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UnknownAn Automated and Fast Sample Preparation Workflow for Laser Microdissection Guided Ultrasensitive Proteomics.
Not available
S-EPMC11067455 | biostudies-literature
ProteomicsFilter aided, single tip (FAST) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus embryos
We report a filter aided, single tip (FAST) method for ultrasensitive proteomic sample preparation. To minimize sample loss and contamination, the method reduces the surface area of the filter to ~0.1 mm2, the total volume of reagents to < 10 μL, and the number of sample transfer steps to two. 25,88...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2022-07-12 | PXD015175 | Pride
Single blastomere Sample preparation Ultrasensitive proteomics 
UnknownAn Ultrasensitive Spatial Tissue Proteomics Workflow Exceeding 100 Proteomes Per Day.
Not available
S-EPMC12828817 | biostudies-literature
ProteomicsUltrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition
Maternal-to-zygotic transition (MZT) is a conserved and fundamental process during which the maternal environment of oocyte transits to the zygotic genome driven expression program, and terminally differentiated oocyte and sperm are reprogrammed to totipotency. Metaphase II (MII) oocytes and zygotes...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-23 | PXD041399 | Pride
Mouse maternal-to-zygotic transition One-cell embryo Metaphase ii (mii) oocyte Ultrasensitive proteome technology 
ProteomicsAn ultrasensitive method for analysis of viral spike N-glycoforms
Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each N-gl...
ORGANISM(S): Bg505 Sosip.664 Md39 Bg505 Sosip.664 Homo Sapiens (ncbitaxon:9606) 
2021-05-12 | MSV000087414 | MassIVE
ProteomicsImproved Single Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry
we report on improved single-cell proteome coverage through the combination of the previously developed Nanodroplet Processing in One Pot for Trace Samples (nanoPOTS) platform with further miniaturization of liquid chromatography (LC) separations and implementation of an ultrasensitive latest-genera...
ORGANISM(S): Homo sapiens (Human) 
2020-02-26 | PXD016921 | Pride
Eclipse Narrow-bore lc Single cell 
ProteomicsUltrasensitive Top-Down Proteomics Analysis of Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform
We developed an ultrasensitive top down platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples), into a top down workflow. We determined that a unique combination of a nonionic detergent DDM with urea as a protein extr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-22 | MSV000084612 | MassIVE
ProteomicsA Data-Dependent Acquisition Ladder for Ultrasensitive (Neuro)Proteomics
We report here a data acquisition strategy that expands the detectable and quantifiable proteome from trace amounts of protein digests using microanalytical capillary electrophoresis (µCE) electrospray ionization (ESI) high-resolution mass spectrometry (HRMS). Data-dependent acquisition (DDA) was pr...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-18 | PXD027428 | Pride
Neuron Neuroscience Proteomics Mouse. Mass spectrometry Capillary electrophoresis Data dependent acquisition 
ProteomicsA nanoLC-MS/MS system using a nonporous reverse phase column for ultrasensitive proteome analysis
The design of proteome projects is often restricted by the available amounts of sample, and thus there has been much effort to date to improve the sensitivity of proteome analyses. We have developed a new ultrasensitive nanoLC-MS system for the proteome analysis of microscale samples using a nonporo...
ORGANISM(S): Homo Sapiens (human) 
2018-09-24 | PXD011168 | JPOST Repository
ProteomicsAn automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomics
Spatial tissue proteomics integrating whole-slide imaging, laser microdissection and ultrasensitive mass spectrometry is a powerful approach to link cellular phenotypes to functional proteome states in (patho)physiology. To be applicable to large patient cohorts and low sample input amounts, includi...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2024-03-22 | PXD047762 | Pride
Spatial proteomics Mass spectrometry Tissue proteomics Sample preparation Robotics 
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