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ProteomicsUniversal plant phosphoproteomics workflow and its application to tomato signaling in response to cold stress
Phosphorylation-mediated signaling transduction plays a crucial role in the regulation of plant defense mechanisms against environmental stresses. To address the high complexity and dynamic range of plant proteomes and phosphoproteomes, we present a universal sample preparation procedure that facili...
ORGANISM(S): Solanum Pimpinellifolium Solanum Lycopersicum 
2018-09-20 | PXD010310 | JPOST Repository
ProteomicsPost-translationally modified and stop codon readthrough of recombinant Francisella tularensis Universal stress protein in Escherichia coli
Recombinant Francisella tularensis universal stress protein with a C-terminal histidine-tag (rUsp/His6) was expressed in Escherichia coli. Endogenous F. tularensis Usp has a predicted molecular mass of 30 kDa, but rUsp/His6 had an apparent molecular weight of 33 kDa based on Western blot analyses. ...
ORGANISM(S): Francisella tularensis subsp. tularensis 
2024-05-24 | PXD045793 | Pride
Escherichia coli pet expression system Acetylation Francisella tularensis universal stress protein Transglutaminase Polyamination Stop codon readthrough 
ProteomicsMass spectrometry distinguishes putrescine, spermidine, and spermine covalently attached to proteins
Bacterial and mammalian cells are rich in putrescine, spermidine and spermine. Polyamines can be incorporated into proteins in vitro. Very few naturally occurring polyaminated proteins have been identified. Bovine albumin and the recombinant universal stress protein from Francisella tularensis we...
ORGANISM(S): Bos taurus (Bovine) 
2024-02-27 | PXD046396 | Pride
Polyamine Universal stress protein Transglutaminase Mass spectrometry Manual evaluation Carbodiimide Albumin 
ProteomicsThe response of Haloferax volcanii to salt and temperature stress: a proteome study by label-free mass spectrometry
We performed an in-depth proteome analysis of the haloarchaeal model organism Haloferax volcanii under standard, low and high salt and low and high temperature conditions using label-free mass spectrometry. Qualitative analysis of protein identification data from high pH/reversed phase fractionated ...
ORGANISM(S): Haloferax volcanii DS2 
2019-09-04 | PXD011056 | Pride
Swath Haloferax volcanii Mass spectrometry Stress response Archaea Proteome 
UnknownA Universal Stress Protein That Controls Bacterial Stress Survival in Micrococcus luteus.
Not available
S-EPMC6872200 | biostudies-literature
UnknownUniversal stress protein Rv2624c alters abundance of arginine and enhances intracellular survival by ATP binding in mycobacteria.
Not available
S-EPMC5071874 | biostudies-literature
UnknownRNA Chaperone Function of a Universal Stress Protein in Arabidopsis Confers Enhanced Cold Stress Tolerance in Plants.
Not available
S-EPMC5751149 | biostudies-literature
TranscriptomicsStress-dependent CHIP/Daxx interaction suppresses the p53 apoptotic program
Our previous studies have implicated CHIP as a co-chaperone/ubiquitin ligase, whose activities yield protection against stress-induced apoptotic events. In this report, we demonstrate a stress-dependent interaction between CHIP (carboxyl terminus of Hsp70-interacting protein) and Daxx, death domain-...
ORGANISM(S): Mus musculus 
2010-04-03 | E-GEOD-14339 | biostudies-arrayexpress
UnknownProtein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation.
Not available
S-EPMC6495262 | biostudies-literature
TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species
B. cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species.
To expose the cultures to heat stress, cells were grown at 37ºC to an O.D. of 0.4 to 0.45 and then transferred into a different shaking incubator at 42.5ºC, incubated for 1 hour at 150 rpm and harvested.<...
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2791 | biostudies-arrayexpress
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