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Glands were frozen at -80 ºC immediately after being retrieved and stored under this condition until initiating the proteomics analysis protocol. Glands were washed with cold PBS and immersed in 120 l of homogenization buffer (50 mM Tris-HCl, pH 6.8, 10 mM DTT, 4% (w/v) SDS). Glands were boiled for ...
ORGANISM(S): Trichonephila clavipes 
2022-12-07 | PXD023141 | Pride
We aimed to characterize the proteome remodeling in DCs induced upon antigen presentation. To do so, we used an in vitro antigen presentation model to generate postsynaptic (psDC) or nonsynaptic (nsDC) DCs similarly to previously reported (Alcaraz-Serna et al., 2021). Bone marrow-derived DCs (BMDCs)...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD039035 | Pride
U2OS cells were either asynchronous or synchronized in late mitosis (anaphase/telophase) prior to irradiation. Asynchronous cells were grown to 75–80% confluence, irradiated with 4 Gy, and collected 5–10 minutes later. For synchronized samples, cells were subjected to a thymidine block, released int...
ORGANISM(S): Homo sapiens (Human) 
2026-03-18 | PXD064722 | Pride
Mucolipidosis type II (ML II) is a rare lysosomal storage disorder caused by deficiency of the UDP-GlcNAc:N-acetylglucosamine-1-phosphotransferase enzyme, which catalyzes the synthesis of the mannose-6-phosphate (M6P) targeting signal for lysosomal acid hydrolases. This deficiency hinders lysosomal ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD060270 | Pride
TaGPC1 and TaGPC2 are NAC-domain transcription factors which accelerate the onset of senescence and facilitate nutrient translocation in wheat. We developed knockout mutants of these genes in tetraploid wheat and used RNA-seq to identify the effect of these mutations on the wheat flag leaf transcrip...
ORGANISM(S): Triticum turgidum subsp. durum 
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