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ProteomicsDefining changes in the total levels of proteins during aging of C. elegans
We performed label-free proteomics in C. elegans to define and quantify changes in the ubiquitinated (Ub)-proteome during the aging process. More specifically, we compared wild-type worms at the first day of adulthood with young (day 5), mid-age (day 10) and aged adults (day 15). Moreover, we assess...
ORGANISM(S): Caenorhabditis elegans 
2021-05-26 | PXD025128 | Pride
C. elegans Proteome Aging 
ProteomicsDefining changes in the steady-state levels of ubiquitinated peptides in aging and longevity
We performed diGLY enrichment followed by label-free proteomics in C. elegans to define and quantify changes in the ubiquitinated (Ub)-proteome during the aging process. More specifically, we compared wild-type worms at the first day of adulthood with young (day 5), mid-age (day 10) and aged adults ...
ORGANISM(S): Caenorhabditis elegans 
2021-05-27 | PXD024338 | Pride
Ubiquitination Aging C. elegans 
ProteomicsDefining changes in the total levels of proteins upon RPN-6 RNAi
Here we defined changes in the total levels of individual proteins upon RPN-6 RNAi in young C. elegans adults (day 5)
ORGANISM(S): Caenorhabditis elegans 
2021-05-26 | PXD024095 | Pride
Aging C. elegans Proteasome Ubiquitin 
ProteomicsImmunoprecipitation of proteins containing Lys48-linked polyUb chains in wild-type C. elegans at day 5 of adulthood
We performed immunoprecipitation experiments in young worms using an antibody against Lys48-linked polyUb followed by single shot label-free proteomics.
ORGANISM(S): Caenorhabditis elegans 
2021-05-26 | PXD024093 | Pride
Aging Proteasome 
ProteomicsDefining changes in the levels of Ub-modified peptides upon rpn-6 knockdown
Here we defined changes in the levels of Ub-modified peptides upon rpn-6 knockdownthat in young C. elegans adults (day 5)
ORGANISM(S): Caenorhabditis elegans 
2021-05-26 | PXD024094 | Pride
Aging Ubiquitination Proteasome 
ProteomicsImmunoprecipitation of proteins containing Lys63-linked polyUb chains in wild-type C. elegans at day 5 of adulthood
We performed immunoprecipitation experiments in young worms using an antibody against Lys63-linked polyUb followed by single shot label-free proteomics.
ORGANISM(S): Caenorhabditis elegans 
2021-05-26 | PXD024045 | Pride
C. elegans Lys63-polyub 
ProteomicsA post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells
While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we ...
ORGANISM(S): Homo sapiens (Human) 
2018-05-16 | PXD007270 | Pride
Shrna Lc-ms Hescs 
ProteomicsCo-immumnoprecipitation experiments to identify protein bindining partners of CSDE1
While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we ...
ORGANISM(S): Homo sapiens (Human) 
2018-05-16 | PXD007271 | Pride
Lc-ms Co-ip Hescs 
ProteomicsIdentification of ubiquitination sites of purified histone H3
Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. When compared with their differentiated counterparts, immortal human embryonic stem cells (hESCs) have a unique chr...
ORGANISM(S): Homo sapiens (Human) 
2020-06-01 | PXD018621 | Pride
Epigenetics Ubiquitination Histone h3 
ProteomicsComparison non-targeting hRNA hESCs versus UBE2K shRNA hESCs
Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. When compared with their differentiated counterparts, immortal human embryonic stem cells (hESCs) have a unique chr...
ORGANISM(S): Homo sapiens (Human) 
2020-06-01 | PXD018625 | Pride
Histones Ubiquitin proteasome system Hescs 
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