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ProteomicsTGFBR2-dependent Alterations of Exosomal Cargo in DNA Mismatch Repair-deficient HCT116 Colorectal Cancer Cells
DNA mismatch repair-deficient colorectal tumors (CRC) frequently show coding microsatellite frameshift mutations in the MSI tumor driver Transforming Growth Factor Beta Receptor Type 2 (TGFBR2) leading to cellular alteration of MSI tumor cells [Lee, J., Warnken, U., Schnölzer, M., Gebert, J. & Kopit...
ORGANISM(S): Homo sapiens (Human) 
2017-04-07 | PXD005620 | Pride
Dna mismatch repair deficiency Lc-msms Transforming growth factor beta receptor type 2 Colorectal cancer Microsatellite instability Exosomes Proteomics 
ProteomicsSplit-TurboID with Bre5 and Rps2 in Saccharomyces cerevisiae
The in vivo labeling technique Split-Biotin identification (Split-BioID, De Munter et al., 2017, Schopp et al., 2017, Cho et al., 2020) was used to capture the common microenvironment of Bre5 and the ribosomal protein Rps2 (uS5). Biotinylated proteins were enriched from cell lysates using affinity p...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-11-10 | PXD028879 | Pride
Split-turboid Rps2 Split-bioid Us5 Silac Saccharomyces cerevisiae Ribosome Bre5 
ProteomicsProxiomes of the greenbeard proteins SmDOC1 and SmDOC2 in the filamentous fungus Sordaria macrospora
In this study, we used the in vivo proximity labeling biotin identification (BioID; Roux et al., 2012) method to capture proteins of the microenvironment of the greenbeard determinant of communication (DOC) proteins SmDOC1 and SmDOC2 in the filamentous fungus Sordaria macrospora. The genes Smdoc1 (S...
ORGANISM(S): Sordaria macrospora 
2026-08-19 | PXD069539 | Pride
Sordaria macrospora Turboid Proximity labeling Bioid Determinant of communication 
UnknownViridistratins A-C, Antimicrobial and Cytotoxic Benzo[j]fluoranthenes from Stromata of Annulohypoxylon viridistratum (Hypoxylaceae, Ascomycota).
Not available
S-EPMC7277860 | biostudies-literature
ProteomicsProximity labeling of the SmSTRIPAK complex in Sordaria macrospora using the SCI1 subunit as bait to identify its SmSTRIPAK-dependent microenvironment
In this study, we used the in vivo proximity labeling biotin identification (BioID; Roux et al., 2012) method to capture proteins of the microenvironment of the striatin interacting kinase and phosphatase complex of the filamentous fungus Sordaria macrospora (SmSTRIPAK). For that, the TurboID biotin...
ORGANISM(S): Sordaria macrospora 
2026-08-19 | PXD069198 | Pride
Sordaria macrospora Turboid Stripak Proximity labeling Bioid 
ProteomicsBacterial Secretion Systems Contribute to Rapid Tissue Decay in Button Mushroom Soft Rot Disease
The soft rot pathogen Janthinobacterium agaricidamnosum causes devastating damage to button mushrooms (Agaricus bisporus), one of the most cultivated and commercially relevant mushrooms. We previously discovered that this pathogen releases the membrane-disrupting lipopeptide jagaricin. This bacteria...
ORGANISM(S): Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628 Agaricus bisporus (White button mushroom) 
2023-07-02 | PXD040852 | Pride
Janthinobacterium agaricidamnosum Secretome analysis Lytic enzymes Type iii secretion Mushroom pathogens 
ProteomicsProxiome at the 40S ribosomal head region according to proximity labeling MS experiments
The in vivo labeling technique Biotin identification (BioID, Roux et al., 2012) was used to capture proteins in the microenvironments of the ribosomal proteins Rps3 (uS3) and Rps20 (us10) in Saccharomyces cerevisiae. Biotinylated proteins were captured from cell lysates using affinity purification a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-11-10 | PXD027267 | Pride
Rps20 Us10 Us3 Silac Saccharomyces cerevisiae Ribosome Rps3 Bira* Bioid 
ProteomicsIn vivo proximity-labeling with miniTurboID to screen for protein-protein interactions in the filamentous ascomycete Sordaria macrospora
The Biotin Identification (BioID) method is an in vivo labeling strategy for the identification of cellular microenvironments in animal cells, plants, yeast and filamentous fungi. With this method, the protein of interest (POI) is fused to a promiscuous biotin ligase. The ligase biotinylates proxima...
ORGANISM(S): Sordaria macrospora 
2025-05-12 | PXD061752 | Pride
Sordaria macrospora Stripak Proximity labeling Miniturbo Miniturboid Bioid 
ProteomicsThe velvet protein Vel1 controls initial plant root colonization and conidia formation for xylem distribution in Verticillium wilt
Velvet domain proteins coordinate developmental processes and secondary metabolism in fungi. In this study, we characterized the role of all four velvet proteins in the life cycle of the plant pathogenic fungus Verticillium dahliae. From other fungi, the formation of different homo-and heterodimers ...
ORGANISM(S): Verticillium dahliae JR2 
2021-02-23 | PXD021861 | Pride
Velvet domain Verticillium dahliae Velvet proteins Plant pathogen 
ProteomicsFungal COP9 signalosome assembly requires connection of two trimeric intermediates for integration of intrinsic deneddylase
The eight-subunit COP9 signalosome (CSN complex), controls the exchange of E3 ubiquitin cullin RING ligase receptors through its deneddylase activity, providing specificity to eukaryotic protein degradation. The conserved eight-subunit CSN complex is required for multicellular development of the fil...
ORGANISM(S): Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) 
2023-08-10 | PXD043205 | Pride
Cop9 signalosome Aspergillus nidulans Gfp-affinity purifications Csn complex Native complex assembly Subcomplexes 
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