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TranscriptomicsDeciphering the transcriptional response mediated by the redox-sensing system HbpS-SenS-SenR from streptomycetes
Comparative analyses of the transcriptomes of the Streptomyces coelicolor A3(2) wild-type and the generated hbpSc-senSc-senRc (hsr) mutant under native and oxidative-stressing conditions allowed to identify differentially expressed genes, whose products may enhance the anti-oxidative defense of the ...
ORGANISM(S): Streptomyces coelicolor 
2016-06-30 | E-MTAB-4833 | biostudies-arrayexpress
TranscriptomicsMonitoring global differential gene expression in context of protein thiol-oxidation and protein S-mycothiolation in Mycobacterium smegmatis str. MC2 155 under hypochlorite stress.
Mycobacterium smegmatis mc2 155 wild type strain was grown in triplicate in modified Hartmans-de Bont minimal medium (HdB) at 37C and vigorous agitation. Cultures were exposed to 1 mM NaOCl at an optical density at 500 nm (OD500) of 0.4 0.45 in the early log phase. M. smegmatis mc2 155 cells of 3...
ORGANISM(S): Mycobacterium smegmatis str. MC2 155 
2016-03-13 | E-MTAB-4522 | biostudies-arrayexpress
TranscriptomicsDifferential transcriptomic analysis reveals hidden light stress response in Streptomyces lividans TK24
By the direct comparison of illuminated and non-illuminated microtiter plate cultures of Streptomyces lividans TK24 transcriptomes, the effects of differing cultivation systems by the relevance of light, a parameter that is usually out of scope during heterotrophic bioprocesses, could be addressed.
ORGANISM(S): Streptomyces lividans 
2017-05-01 | E-MTAB-5623 | biostudies-arrayexpress
TranscriptomicsMonitoring global differential gene expression in Corynebacterium glutamicum in response to sigma factor SigD disruption and overexpression
In this study, disruption and overexpression of sigD were performed in Corynebacterium glutamicum and analyzed by transcriptome sequencing (RNA-seq) to understand the SigD regulon in C. glutamicum. For the effect of sigD overexpression, the relative abundance of mRNA was compared in WT(pVWEx1-sigD) ...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2017-01-27 | E-MTAB-5411 | biostudies-arrayexpress
TranscriptomicsThe guanidinobutyrase GbuA is essential for the alkylquinolone-regulated pyocyanin production during parasitic growth of Pseudomonas aeruginosa in co-culture with Aeromonas hydrophila
The opportunistic pathogen Pseudomonas aeruginosa controls the production of virulence factors via its quorum sensing (QS) systems. The addition of 100 M 4-GB to succinate-containing medium led to a strong reduction of pyocyanin production in strain PAO1gbuA, whereas pyocyanin production by the wild...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2016-06-08 | E-MTAB-4777 | biostudies-arrayexpress
TranscriptomicsThe global effect of the garlic compound Allicin on the changes in the transcriptome in Bacillus subtilis and Staphylococcus aureus USA300 analysed by RNA-seq of coding RNA
To monitor the global changes in gene expression of Staphylococcus aureus USA300 TCH1516 under exposure to the garlic compound Allicin by RNA-seq, cultivation was performed in shaking flasks in Luria Bertani (LB) medium in triplicate at 37C until cells have reached an optical density at 540nm of 2.0...
ORGANISM(S): Staphylococcus aureus subsp. aureus USA300 
2019-04-01 | E-MTAB-5667 | biostudies-arrayexpress
TranscriptomicsThe global effect of hypochlorite stress on the changes in the transcriptome in Staphylococcus aureus USA300
To monitor the global changes in gene expression of Staphylococcus aureus USA300 TCH1516 under hypochlorite stress by RNA-seq, cultivation was performed in Luria Bertani (LB) medium in triplicate at 37C until cells have reached an optical density at 540nm of 2.0. Cells were harvested by centrifugat...
ORGANISM(S): Staphylococcus aureus subsp. aureus USA300 
2017-12-18 | E-MTAB-5666 | biostudies-arrayexpress
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