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ProteomicsQualitative and quantitative top-down proteomics of human colorectal cancer cell lines identified 23000 proteoforms and revealed drastic proteoform-level differences between metastatic and non-metastatic cancer cells
Understanding cancer metastasis at the proteoform level is crucial for discovering new protein biomarkers for cancer diagnosis and drug development. Proteins are the primary effectors of function in biology and proteoforms from the same gene can have drastically different biological functions. Here,...
ORGANISM(S): Homo sapiens (Human) 
2023-01-05 | PXD029703 | Pride
Top-down proteomics Proteoform Cancer metastasis Colorectal cancer 
ProteomicsA pilot study for deciphering post-translational modifications and proteoforms of tau protein by capillary electrophoresis-mass spectrometry
Abnormal accumulation of tau proteins is one pathological hallmark of Alzheimer’s disease (AD). Many tau protein post-translational modifications (PTMs) are associated with the development of AD, such as phosphorylation, acetylation, and methylation. Therefore, a complete picture of PTM landscape of...
ORGANISM(S): Escherichia coli 
2024-10-15 | PXD053367 | Pride
Alzheimer’s disease Capillary electrophoresis-mass spectrometry Post-translational modification Tau protein Phosphorylation Proteoform 
ProteomicsAn improved capillary isoelectric focusing-mass spectrometry method for characterization of monoclonal antibody charge variants
Here, we improved the stability and enhanced separation resolution of automated cIEF-MS by bettering the quality of capillary neutral coating, reducing catholyte pH to 10 for cIEF-MS for the first time, and systematically optimizing the cIEF separation conditions for characterization of monoclonal a...
ORGANISM(S): Homo sapiens (Human) 
2026-08-19 | PXD082799 | Pride
Monoclonal antibody charge variants Cief-ms 
ProteomicsNanoparticle-aided nanoreactor for large-scale proteomics of few mammalian cells
Large-scale bottom-up proteomics of few even single cells is crucial for a better understanding of the roles played by cell-to-cell heterogeneity in disease and development. Novel proteomic methodologies with extremely high sensitivity are required for few even single-cell proteomics. Sample process...
ORGANISM(S): Homo sapiens (Human) 
2022-07-11 | PXD027135 | Pride
Nanoreactor Single-cell proteomics Mass-limited sample Nanoproteomics Bottom-up proteomics Nanoparticle Few-cell proteomics 
ProteomicsPredicting electrophoretic mobility of proteoforms for large-scale top-down proteomics
Large-scale top-down proteomics characterizes proteoforms in cells globally with high confidence and high throughput using reversed-phase liquid chromatography (RPLC)-tandem mass spectrometry (MS/MS) or capillary zone electrophoresis (CZE)-MS/MS. The false discovery rate (FDR) from the target-decoy ...
ORGANISM(S): Escherichia coli 
2020-02-17 | PXD017265 | Pride
Top-down proteomics Electrophoretic mobility Cze-ms Proteoform 
ProteomicsAutomated capillary isoelectric focusing-tandem mass spectrometry for top-down proteomics
Mass spectrometry (MS)-based top-down proteomics (TDP) delineates proteoforms in cells and requires high-resolution proteoform separation. Herein, for the first time, we employed an automated capillary isoelectric focusing-tandem MS (cIEF-MS/MS) system for large-scale TDP of complex proteomes. Singl...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Escherichia coli 
2020-12-08 | PXD020342 | Pride
Endogenous hormone proteoforms/gel-free 2d-page/multi-level proteomics/ sexual dimorphism/zebrafish brain 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins
Mass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we ...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-07 | PXD042298 | Pride
Top-down proteomics  integral membrane protein  proteoform  capillary zone electrophoresis-mass spectrometry  mouse brain 
ProteomicsBlocking antibiotic resistance contamination dissemination to neutralized the impacts of bacterial oxidative stress via efficient photocatalysis
Antibiotic resistance genes (ARGs) transmission in wastewater, seriously threaten the ecological safety and human health, which attracted increasing attention throughout the world. However, the correlations between bacterial interaction and environmental stress in photo-inactivation are rarely studi...
ORGANISM(S): Wastewater Metagenome 
2025-08-19 | PXD067488 |
ProteomicsCapillary Zone Electrophoresis-Electron-Capture Collision-Induced Dissociation on a Quadrupole Time-of-Flight Mass Spectrometer for Top-Down Characterization of Intact Proteins
Mass spectrometry (MS)-based denaturing top-down proteomics (dTDP) requires high-capacity separation and extensive gas-phase fragmentation of proteoforms. Herein, we coupled capillary zone electrophoresis (CZE) to electron-capture collision-induced dissociation (ECciD) on an Agilent 6545 XT quadrupo...
ORGANISM(S): Bos taurus (Bovine) Equus caballus (Horse) 
2023-01-05 | PXD030373 | Pride
Electron-capture collision-induced dissociation Top-down Capillary zone electrophoresis 
ProteomicsAqueous humour proteomic analysis of acute angle-closure glaucoma with visual field loss
original data of Aqueous humour proteomic analysis of acute angle-closure glaucoma with visual field loss.
ORGANISM(S): Homo Sapiens 
2021-02-05 | PXD024028 |
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