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ProteomicsDiversity matters: Optimal collision energies for tandem mass spectrometric analysis of a large set of N-glycopeptides - part 2
a) Ratio of the lower and higher energy component. Tests were done on the mixture of AGP, fetuin and transferrin digests using 2.2 pmol from all glycoproteins in each run. Five different high CE setting was used which were 50%, 75%, 100%, 125% and 150% of the original method of Hinneburg et al. Thes...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606) 
2022-08-26 | MSV000090218 | MassIVE
ProteomicsDiversity matters: Optimal collision energies for tandem mass spectrometric analysis of a large set of N-glycopeptides
Detailed energy dependence investigations were carried out on AGP digest using 2.2 pmol glycoprotein per run and on the mixture of AGP, fetuin and transferrin digests injecting 2.2 pmol from all glycoproteins in each run. Stepped CE settings were applied with 80% of the time allocated to the higher ...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606) 
2022-06-15 | MSV000089657 | MassIVE
UnknownEvaluation of Immune-Related Response Criteria and RECIST v1.1 in Patients With Advanced Melanoma Treated With Pembrolizumab.
Not available
S-EPMC5070547 | biostudies-literature
GenomicsA Genomic History of Aboriginal Australia
The population history of Aboriginal Australians remains largely uncharacterised. We generated high-coverage genomes for 83 Aboriginal Australians (speakers of Pama-Nyungan languages) and 25 Papuans from the New Guinea Highlands. We find that Papuan and Aboriginal Australian ancestors diversified 25...
EGAS00001001766 | EGA
TranscriptomicsTranscriptome wide analysis of classically and alternatively activated macrophages
Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived human M1- and M2-like macrophage profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods an...
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-36952 | biostudies-arrayexpress
ProteomicsInteraction mapping of human condensin I and condensin II complexes using DSBU-cross-linking and mass spectrometry
Human condensin I and condensin II complexes play essential roles in chromatin condensation during the cell division process. Both complexes use ATP as the energy source to pack extended chromatin into the short form of chromosome. In order to understand the mechanism of their function, we performed...
ORGANISM(S): Homo sapiens (Human) 
2020-05-25 | PXD017531 | Pride
Condensin Dsbu Q-exactive Cross-linking Human Protein complex Xl-ms Chromatin Chromosome 
ProteomicsCan we boost N-glycopeptide identification confidence? Smart collision energy choice taking into account structure and search engine
We investigated how the structural features of N-glycopeptides and the choice of the search engine influence the optimal collision energy, delivering highest identification confidence. We carried out LC-MS/MS measurements using a series of collision energies on a large set of N-glycopeptides with bo...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606) 
2023-10-14 | MSV000093110 | MassIVE
ProteomicsAnalalysis of ASB6-UBA3-NAE1 interaction
Validation of ASB6 and NAE1-UBA3 interaction using SH tagged cell lines
ORGANISM(S): Homo sapiens (Human) 
2021-01-29 | PXD022175 | Pride
Asb6 pulldown Dda and prm 
TranscriptomicsTranscriptome wide analysis of untreated, classically and alternatively activated macrophages
Analysis of human M0-, M1-, and M2-like macrophages. Total RNA was isolated from untreated, classically and aternative activated human macrophages
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-35449 | biostudies-arrayexpress
UnknownPho84/GLUT2 are cellular myo-inositol exporters regulated by inositol pyrophosphates
Not available
S-SCDT-10_1038-S44318-026-00888-9 | biostudies-other
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