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ProteomicsCross-Link of inactive PhK and GP
Phosphorylase kinase (PhK) phosphorylates GP to initiate glycogenolysis. To gain insights into the mechanism of PhK catalyzing GP phosphorylation, we have tried a variety of methods to obtain the hPhK-GP complex, but none of them were successful. Eventually, we decided to employ XL-MS to investigate...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD059356 | Pride
Phk Gp Xl-ms 
ProteomicsConfirmation of the phosphorylation sites of human PhK
The activity of PhK is regulated by phosphorylation, and we utilized single-particle cryo-electron microscopy to solve the high-resolution structures of human PhK under phosphorylation and calcium ion activation. To gain a deeper insight into the phosphorylation sites following PKA treatment, we emp...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD059345 | Pride
Phosphorylation sites，phk 
ProteomicsCross-Link of active PhK and GP
Phosphorylase kinase (PhK) phosphorylates GP to initiate glycogenolysis. To gain insights into the mechanism of PhK catalyzing GP phosphorylation, we have tried a variety of methods to obtain the hPhK-GP complex, but none of them were successful. Eventually, we decided to employ XL-MS to investigate...
ORGANISM(S): Hordeum vulgare (Barley) 
2025-05-07 | PXD059369 | Pride
Phk Active state Gp Xl-ms 
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