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ProteomicsUbiquitination sites on MIDN identified by mass spectrometry analysis
In E. coli, the pACYC-HA-Ub-E1 (UBA1)-UbcH5A-RNF126 and pET-22b-MIDN-His₆ plasmids were co-transformed. After IPTG induction to express all proteins required for ubiquitination, His-tagged MIDN was enriched with Ni-NTA resin and subjected to mass-spectrometric mapping of its ubiquitin-modified sites...
ORGANISM(S): Homo sapiens (Human) 
2026-06-01 | PXD069387 | Pride
Ubiquitination sites Midn 
ProteomicsIdentification of p62-interacting proteins by mass spectrometry analysis in HEK293T cells
HEK293T cells were ectopically expressing Flag tagged p62 (p62 group) or empty vectors (Control group) for 48h, cell lysates were incubated with anti-Flag affinity gels, and co-immunoprecipitates were subjected to trypsin digestion followed by mass spectrometry analysis. Peptides were separated by t...
ORGANISM(S): Homo sapiens (Human) 
2025-07-29 | PXD062511 | Pride
Hek293t cells P62 Mass spectrometry analysis 
ProteomicsIdentification of p62-interacting proteins by mass spectrometry analysis in HEK293T cells
HEK293T cells were ectopically expressing Flag tagged p62 (p62 group) or empty vectors (Control group) for 48h, cell lysates were incubated with anti-Flag affinity gels, and co-immunoprecipitates were subjected to trypsin digestion followed by mass spectrometry analysis. Peptides were separated by t...
ORGANISM(S): Homo sapiens (Human) 
2025-07-29 | PXD061794 | Pride
Hek293t cells P62 Mass spectrometry analysis 
UnknownEpidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021.
Not available
S-EPMC9146497 | biostudies-literature
TranscriptomicsExpression data from testes of CG3875 knockout and wildtype flies
CG3875 is a young duplicate gene of kep1 family originated recently in Drosophila melanogaster (D. melanogaster) species complex (including D. melanogaster, D. simulans, D. mauritiana and D. sechellia) after it split from D. yakuba. It encodes an RNA-binding protein containing a single maxi-KH domai...
ORGANISM(S): Drosophila melanogaster 
2010-12-31 | E-GEOD-22289 | biostudies-arrayexpress
UnknownFloral Developmental Morphology and Biochemical Characteristics of Male Sterile Mutants of <i>Lagerstroemia indica</i>.
Not available
S-EPMC11548521 | biostudies-literature
UnknownIdentification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China.
Not available
S-EPMC11209087 | biostudies-literature
TranscriptomicsBacillus subtilis 168, WT vs. ΔMWX, WT vs. Δ7ECF, Δ7ECF vs. ΔMWX.
Bacillus subtilis encodes seven extracytoplasmic function (ECF) sigma factors. Three (sigma M, sigma W and simga X) mediate responses to cell envelope active antibiotics. The functions of sigma Y, sigma Z, sigma V, and YlaC remain largely unknown, and strong inducers of these sigma factors and their...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2010-07-21 | E-GEOD-22930 | biostudies-arrayexpress
UnknownRelationships between cell cycle pathway gene polymorphisms and risk of hepatocellular carcinoma.
Not available
S-EPMC4917616 | biostudies-literature
UnknownKnockdown resistance (kdr) mutations within seventeen field populations of Aedes albopictus from Beijing China: first report of a novel V1016G mutation and evolutionary origins of kdr haplotypes.
Not available
S-EPMC6480817 | biostudies-literature
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