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ProteomicsAP-MS in CRISPR/Cas9 Utilizing Systems for Mapping Endogenous Protein Complexes
We combined clustered regularly interspaced short palindromic repeats/Cas9-mediated gene knockout technology with affinity purification using antibodies against endogenous proteins followed by mass spectrometry analysis, to sensitively and precisely detect protein-protein interactions in unaltered i...
ORGANISM(S): Homo sapiens (Human) 
2017-03-15 | PXD005107 | Pride
Ap-ms Acumen Nf1 Crispr/cas9 Kras 
ProteomicsRecoding multiple rare codons enables the simultaneous incorporation of up to five distinct noncanonical amino acids
Expanding the genetic code has revolutionized our ability to study and manipulate biological systems through site-specific incorporation of noncanonical amino acids (ncAAs). However, current methods are primarily limited to single-type ncAA incorporation in mammalian cells owing to translation ineff...
ORGANISM(S): Homo Sapiens 
2025-11-04 | PXD070277 |
MetabolomicsAberrant gut microbiota alters host metabolome and impacts renal failure in humans and rodents

OBJECTIVE: Patients with renal failure suffer from symptoms caused by uraemic toxins, possibly of gut microbial origin, as deduced from studies in animals. The aim of the study is to characterise relationships between the intestinal microbiome composition, uraemic toxins and rena...

2020-10-20 | MTBLS700 | MetaboLights
ProteomicsRaw data of 218 FFPE tissue proteomes containing 144 tissue samples from seven organs of fatal VID-19 cases and 74 control specimens from 56 non-COVID-19 individuals
144 tissue samples from postmortem lung, liver, kidney, heart, spleen, testis and thyroid collected from 19 COVID-19 cases and 74 control specimens from 56 non-COVID-19 individuals were analyzed by PCT and TMT 16-plex based proteomics.
ORGANISM(S): Homo Sapiens 
2020-12-11 | PXD022775 |
UnknownUsing CSF biomarkers to replicate genetic associations in Alzheimer's disease.
Not available
S-EPMC3150628 | biostudies-literature
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