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Pasteurized whole cow milk (Grade A) was purchased from the supermarket. Whole cow milk was centrifuged at 3 000 g at 4°C for 30 min and the fat layer was recovered. The recovered fat was washed with PBS for 20 min at 37°C, and the resuspened mixture was centrifuged again to obtain the milk fat. The...
ORGANISM(S): Bos taurus (Bovine) 
2018-10-19 | PXD009288 | Pride
Wild-type and delta-rpoE strains of H:z66 positive S. Typhi were grown respectively in 1 ml of LB broth (pH 7.0) at 37oC with shaking (200 rpm) overnight. 200 ul of each culture was then transfer-incubated in fresh 20 ml of LB broth to log phase (0.5 OD at 600 nm). Ampicillin was added in cultures t...
ORGANISM(S): Salmonella typhi GIFU10007 

This study aimed to investigate fecal metabolomic profiles associated with osteoporosis and serum S-equol levels in community-dwelling older adults from a tropical region of China. Fecal samples were collected from participants classified as osteoporosis or non-osteoporosis based on bone mineral ...

2026-02-10 | MTBLS13855 | MetaboLights
Hypusine is a post-translational modification on eukaryotic translation initiation factor 5A (eIF5A). The last step in the biosynthesis of hypusine, deoxyhypusine hydroxylation, is an oxygen dependent reaction. Here we show that deletion of deoxyhypusine hydroxylase, Lia1, compromises yeast respirat...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-12 | PXD032321 | Pride
The phosphorylation modification of protein LFRQ was investigated by LC-MS/MS.
ORGANISM(S): Candida albicans (Yeast) 
2024-03-15 | PXD037938 | Pride
Extracellular vesicles (EV) as drug delivery nanocarriers are under intense investigation. Although clinical-grade EVs have been produced on a large-scale, low yield and high production costs of natural EVs (nEV) limit the relevant industrial translation. Recent studies show that mechanical extrusio...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD030826 | Pride
Identificaiton of bacterial chemoreceptors by DSSO-based MS2-MS3 method.
ORGANISM(S): Escherichia coli 
2021-09-09 | PXD019599 | Pride
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