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Extracellular vesicles play crucial roles in intercellular communication in tumor microenvironment. Here, we demonstrate that in hepatic fibrosis, TGF-β stimulates the palmitoylation of hexokinase 1 (HK1) in hepatic stellate cells (HSCs), which facilitates the secretion of HK1 via large extracellula...
ORGANISM(S): Homo Sapiens 
2022-08-09 | PXD035911 |
We used SPIDER to examine the cell-surface receptors for specific ligands while these receptors were in their native membranes within intact cells in culture. Here we coupled the SPIDER assay with biotinylated RBD of the Omicron Variant of SARS-CoV-2 and biotinylated RBD of SARS-CoV-2. Then we perfo...
ORGANISM(S): Homo Sapiens Chlorocebus Sabaeus 
2022-01-18 | PXD031035 |
Characterization of GFP-Pup after Pupylation assays by mass spectrometry.
ORGANISM(S): Mycobacterium Tuberculosis H37rv_2009 
We used CheAs and biotin-CheZ to validate the efficiency of SPIDER capturing protein-protein interaction. Pupylation sites on WT CheAs after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e.,...
ORGANISM(S): Escherichia Coli 
Characterization of GFP-Pup before or after pupylation assays by Thermo Exactive Plus EMR mass spectrometry coupled to Agilent 1100 HPLC system
ORGANISM(S): Mycobacterium Tuberculosis H37rv_2009 
2021-06-04 | PXD026527 |
To assess whether SPIDER could detect transient interactions such as enzymes and their substrates, we examined the interactome of the E. coli protein deacetylase CobB. As the only member of the Sir2 family of deacetylases in E. coli, CobB is known to play a role in many different pathways but their ...
ORGANISM(S): Escherichia Coli 
As a test for the effectiveness of SPIDER with RNA-protein pairs, We used Sox2 and specific biotin-DNA and biotin-RNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on Sox2 after SPIDER assay was identified by LC-MS/MS analysis, by searching for an ...
ORGANISM(S): Homo Sapiens 
2021-06-04 | PXD026517 |
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