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ProteomicsCombination of multi-enzyme digestions and C-TAILS approach for deep screening of C-terminome
Protein C-termini study is still a challenging task and far behind its counterpart N-termini study. The identification of C-termini is often hampered by low ionization response in MS and fewer efficient enrichment methods existing. We previously optimized the C-TAILS (C-terminal amine-based isotope ...
ORGANISM(S): Escherichia coli 
2017-11-24 | PXD004275 | Pride
Protein c-termini C-tails Multi-enzyme Scii 
ProteomicsSystematic optimization of C-TAILS approach for deep screening of C-terminomics
It is well known that protein C-termini play important roles in various biological processes, and thus the precise characterization of C-termini is essential for fully elucidating protein structure and understanding protein functions. Although many efforts have been made in the fields during the lat...
ORGANISM(S): Escherichia coli 
2015-09-15 | PXD002409 | Pride
Protein c-termini Acetylation Scii 
ProteomicsLys-C/Arg-C, a New Enzyme Combination for Next-Generation Proteomics
Today, the most widely used bottom-up proteomics studies necessitate a proteolysis step prior to MS analysis. Trypsin is often the best protease in choice due to its high specificity and MS-favored proteolytic products. Trypsin has been challenged for its low cleavage efficiency at Lys-X bonds, and ...
ORGANISM(S): Homo sapiens (Human) 
2018-07-24 | PXD009797 | Pride
Lys-c / arg-c / trypsin / dia / mass spectrometry 
ProteomicsShort-term mild temperature-stress-induced alterations in C. elegans phosphoproteome
Exposure to mild early-life stresses can slow down the aging process, in which protein phosphorylation might be an essential regulator. Currently, the understanding of phosphorylation-based regulatory networks under mild early-life stress remains elusive. Herein, we systematically analyzed the phosp...
ORGANISM(S): Caenorhabditis elegans 
2020-09-17 | PXD021081 | Pride
Phosphoproteomics  c. elegans  mild temperature  lifespan  mild stresses 
ProteomicsQuantitative temporal phosphoproteomics of aging C.elegans under different environmental temperatures
Temperature is one of the primary environmental factors that affect aging, in which protein phosphorylation is an important regulator. Currently, the understanding of phosphorylation events in regulatory networks during aging has remained rather limited. Therefore, the phosphoproteomes of C.elegans ...
ORGANISM(S): Caenorhabditis elegans 
2018-08-08 | PXD009661 | Pride
C.elegans  phosphoproteomics  itraq  natural aging  accelerated aging 
ProteomicsAn Undesirable Side Effect of EDC-catalyzed Carboxyl Labeling Approach
Carbodiimide catalyzed carboxyl and amine conjugation (amidation) has been widely used to protect carboxyl groups. N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) is the most commonly used carbodiimide reagent in protein chemis-try due to its high efficiency and high solubility in aqueous media...
ORGANISM(S): Homo sapiens (Human) 
2020-12-10 | PXD020042 | Pride
Tyrosine Carboxyl protection C-tails Edc 
Proteomics102-Plex Approach for Accurate and Multiplexed Proteome Quantification
Numerous hyperplexing approaches have been developed to meet the demand for large-scale basic and clinical analysis. Currently, the analysis capacity has expanded to up to 54 samples within a single experiment by utilizing different isotopic and isobaric reagent combinations. In this report, we prop...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-01-15 | PXD042398 | Pride
Multiplexing Quantitative proteomic analysis 102-plex approach 
ProteomicsSP3-PELSA: A Rapid and Sensitive Approach for Studying Protein-Ligand Interaction with Limited Samples
SP3-PELSA, a modified peptide-centric local stability assay integrated with SP3 sample preparation, enables fivefold faster processing, enhanced peptide recovery (especially for hydrophobic peptides and low-amount samples), and superior performance in drug target discovery, holding great potential f...
ORGANISM(S): Homo Sapiens 
2025-12-10 | PXD071732 |
ProteomicsBasic Strong Cation Exchange Chromatography, BaSCX, a Highly Efficient Approach for C-Terminomic Studies Using LysargiNase Digestion
Decoding protein C-termini is a challenging task in protein chemistry using conventional chemical/enzymatic approaches. With the rapid development in modern mass spectrometer, many advanced mass spectrometry (MS) based protein C-termini analysis approaches have been established. Although great progr...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Caenorhabditis elegans 
2020-02-28 | PXD016317 | Pride
C-terminomics / lysarginase / scx / bascx / mass spectrometry 
ProteomicsEnzyme and Chemical Assisted N-Terminal Blocked Peptides Analysis, ENCHANT, as a Selective Proteomics Approach Complementary to Conventional Shotgun Approach
Shotgun (bottom-up) approach has been widely applied in large-scale proteomics studies. The inherent shortages of shotgun approach lie in that the generated peptides often overwhelm the analytical capacity of current LC-MS/MS systems and that abundant proteins often hamper the identification of prot...
ORGANISM(S): Homo sapiens (Human) 
2017-11-30 | PXD007863 | Pride
Dia Single-charge ion inclusion N-terminal blocked peptides Cnbr-activated sepharose 4b 
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