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ProteomicsRegulation of the Endocytosis and Prion Chaperoning Machineries by Yeast E3 Ubiquitin Ligase Rsp5 as Revealed by Orthogonal Ubiquitin Transfer
Attachment of the ubiquitin (UB) peptide to proteins via the E1-E2-E3 enzymatic machinery regulates diverse biological pathways, yet identification of the substrates of E3 UB ligases remains a challenge. We overcame this challenge by constructing an “orthogonal UB transfer (OUT) cascade with yeast E...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-13 | PXD023688 | Pride
Ubiquitin Lc-ms/ms 
UnknownIdentifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer.
Not available
S-EPMC5738348 | biostudies-literature
ProteomicsIdentifying the Ubiquitination Targets of E6AP by Orthogonal Ubiquitin Transfer
E3 ubiquitin (UB) ligases, the ending module of the E1-E2-E3 cascades, transmit diverse signals in the cell by attaching UB to cellular proteins. Identifying E3 substrates holds the key to elucidate the roles of E3s in cell regulation. We constructed an orthogonal UB transfer (OUT) cascade to identi...
ORGANISM(S): Homo sapiens (Human) 
2018-02-07 | PXD005584 | Pride
Ubiquitin Iap Lc-ms/ms Conjugate 
UnknownA hierarchical vascularized engineered bone inspired by intramembranous ossification for mandibular regeneration.
Not available
S-EPMC9217949 | biostudies-literature
ProteomicsEngineering a Cell-Based Orthogonal Ubiquitin Transfer Cascade for Profiling the Substrates of RBR E3 Parkin
The E3 ubiquitin (UB) ligase Parkin utilizes a Ring-Between-Ring (RBR) domain to mediate UB transfer to substrate proteins, and mutations affecting Parkin catalysis promote cancer and are associated with Parkinson’s disease. An essential role of Parkin is to initiate mitophagy by ubiquitinating mito...
ORGANISM(S): Homo sapiens (Human) 
2025-07-28 | PXD063080 | Pride
Ubiquitin Parkin Mass spectrometry E3 
ProteomicsPhosphoproteomic Profiling Reveals PI Pathway and Transcription Factor Regulation in Tamarix hispida under Salt Stress
Here, we identify the salt-sensitive proteins in vivo under 200 mM NaCl treatment in Taramix hispida by 4D label free-based phosphoproteomic analysis. Through a normalization by proteomics analysis to phosphorylation omics, 5,425 phosphorylated sites were identified in 1,960 comparable proteins. Mea...
ORGANISM(S): Tamarix Hispida 
2025-06-06 | PXD064704 |
UnknownProtein Engineering in the Ubiquitin System: Tools for Discovery and Beyond.
Not available
S-EPMC7047443 | biostudies-literature
UnknownProfiling and verifying the substrates of E3 ubiquitin ligase Rsp5 in yeast cells.
Not available
S-EPMC10440593 | biostudies-literature
UnknownRegulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer.
Not available
S-EPMC8380759 | biostudies-literature
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