Sort   by:  
 Page size 
ProteomicsRobust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting
Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d. × 150 mm column and at a flow rate of 50 µl/min f...
ORGANISM(S): Homo sapiens (Human) 
2021-02-22 | PXD023650 | Pride
Micro-flow lc Plasma Proteomics Urine 1.0 mm id column 
ProteomicsMicro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) 
2021-07-01 | PXD023587 | Pride
Single shot proteomics Micro-flow lc 1.0 mm id column 
ProteomicsHigh-flow chromatography for reproducible and high-throughput quantification of proteomes
This study evaluates liquid-chromatography operated at 50 ul/min using 1 mm diameter columns coupled online to tandem mass spectrometry (LC-MS/MS) for proteome analysis. Data from >1,000 human cell line, tissue, body fluid, affinity- and phospho-proteomes demonstrate excellent chromatographic (<0.3 ...
ORGANISM(S): Homo sapiens (Human) 
2019-12-09 | PXD015087 | Pride
High-flow lc Chromatography Proteomics Body fluids Kinobeads 
ProteomicsComparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD024169 | Pride
Single shot proteomics Nano-flow lc Micro-flow lc 
ProteomicsITRAQ of HEK293T-cell protein 48 hours after transfection of phagocytophilic anaplastic effector protein Ats-1
ITRAQ of HEK293T-cell protein 48 hours after transfection of phagocytophilic anaplastic effector protein Ats-1
ORGANISM(S): Homo Sapiens 
2021-10-28 | PXD029412 |
ProteomicsTransketolase promotes MAFLD by limiting inosine-induced mitochondrial activity (Proteomics)
Metabolic dysfunction-associated fatty liver disease (MAFLD) has a global prevalence of about 25% and no approved therapy. Using metabolomic and proteomic analyses, we identified high expression of hepatic transketolase (TKT), a metabolic enzyme of the pentose phosphate pathway, in human and mouse M...
ORGANISM(S): Homo Sapiens 
2024-02-26 | PXD050130 |
Sort   by:  
 Page size