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ProteomicsQuantitative analysis of temporal IP and cell lysate using SWATH-MS (2)
We treated wildtype and RIP3 knockout L929 with TNF for 8 time points respectively, and used SWATH-MS to quantify dynamic proteomic change of these two cell lines under a serial TNF treatments.
ORGANISM(S): Mus musculus (Mouse) 
2021-01-11 | PXD002178 | Pride
Mouse L929 cells Swath-ms 
ProteomicsQuantitative analysis of temporal IP and cell lysate using SWATH-MS (1)
We treated L929 cells with TNF for six time points and the samples containing TNFR1 complexes were collected by affinity purification and analyzed by SWATH-MS. Accurate and consistent quantification across 18 IP samples showed dynamic profiles of the TNFR1 complex components across six time points.
ORGANISM(S): Mus musculus (Mouse) 
2021-01-11 | PXD002177 | Pride
Mouse L929 cells Swath-ms 
Proteomicsa novel SDS-based sample preparation for proteomics
Sodium dodecyl sulfate (SDS) is widely used in sample preparation for proteomic research. However, SDS is incompatible with liquid chromatography and electrospray ionization (ESI). SDS depletion is therefore required ahead of liquid chromatography coupled with mass spectrometry (LC-MS) analysis. Mos...
ORGANISM(S): Homo Sapiens Mus Musculus 
2021-03-02 | PXD023557 |
ProteomicsdiaPASEF analysis of exosomes from stem cells
we used highly sensitive mass spectrometry diaPASEF (parallel accumulation serial fragmentation combined with the data-independent acquisition)27 to quantify proteins of exosomes derived from umbilical cord mesenchymal stem cells (UCMSCs) and rat bone marrow stem cells (BMSCs). 4206 human proteins a...
ORGANISM(S): Homo Sapiens Rattus Norvegicus 
2022-05-05 | PXD033723 |
ProteomicsEvaluation of library-free strategies for phosphoproteomics and ubiquitinomics data-independent acquisition data
Phosphoproteomics and ubiquitinomics data-independent acquisition MS data is generally analyzed using a DDA spectral library. Performance of different library-free strategies of analyzing phosphoproteomics and ubiquitinomics DIA MS data are not evaluated. In this study, we assess three library-free ...
ORGANISM(S): Homo Sapiens 
2023-05-17 | PXD037870 |
ProteomicsElevated NAD+ drives Sir2A-mediated GCβ deacetylation and localization for Plasmodium ookinete gliding and mosquito infection
cGMP signal-activated ookinete gliding is essential for mosquito midgut infection of Plasmodium in malaria transmission. During ookinete development, cGMP synthesizer GCβ polarizes to a unique localization “ookinete extrados site” (OES) until ookinete maturation and activates cGMP signaling for init...
ORGANISM(S): Plasmodium Yoelii Yoelii 17xnl 
2024-09-26 | PXD056305 |
ProteomicsInner membrane complex proteomics reveals a palmitoylation cascade regulating intraerythrocytic development of malaria parasite
Malaria is caused by infection of the erythrocytes by the parasites Plasmodium. Inside the erythrocytes, the parasites grow and multiply via schizogony, an unconventional cell division mode. The Inner Membrane Complex (IMC), an organelle located beneath the plasma membrane of the parasites, is essen...
ORGANISM(S): Plasmodium Yoelii Yoelii 17xnl 
2022-07-01 | PXD028193 |
ProteomicsApical anchorage and stabilization of subpellicular microtubules by apical polar ring ensures Plasmodium ookinete infection in mosquito
Morphogenesis of many protozoans depends on a polarized establishment of cortical cytoskeleton possessing the subpellicular microtubules (SPMTs), which are apically nucleated and anchored by the apical polar ring (APR). In the malaria parasite Plasmodium, APR can be observed when a round zygote diff...
ORGANISM(S): Plasmodium Yoelii Yoelii 17xnl 
2022-11-18 | PXD038209 |
ProteomicsMurine osteoblast, supernatant and serum mass spectrometry analysis
Mass spec data of murine osteoblast, serum and supernatant
ORGANISM(S): Mus Musculus 
2024-07-25 | PXD054258 |
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