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Firstly, wild type seedlings that had germinated in root tubes for 14 days were taken to prepare protoplasts. The GCN5-FLAG transient expression vector was transformed into wild type protoplasts. The protoplasts were collected and purified by Flag beads. After quality testing of the samples, phospho...
ORGANISM(S): Oryza sativa (Rice) 
2025-10-28 | PXD066361 | Pride
Elevated temperatures due to global warming seriously threaten crops production. Understanding molecular mechanisms of cellular responses to heat stress will help to improve crop tolerance to high temperature and yield. In this work, we show that deacetylation of non-histone proteins mediated by the...
ORGANISM(S): Oryza sativa (Rice) 
2024-09-04 | PXD053060 | Pride
These arrays are used for various projects Experiment Overall Design: HG-U133A and HG-U133B data are combined and analyzed together with other U133A & B or with HG-U133plus2 samples. No replicates were performed. Controls are human bronchial epithelial cells (HBECs)
ORGANISM(S): Homo sapiens 
Samples of oil and production water were collected from five wells of the Qinghai Oilfield, China, and subjected to GeoChip hybridization experiments for microbial functional diversity profiling. Unexpectedly, a remarkable microbial diversity in oil samples, which was higher than that in the corresp...
ORGANISM(S): uncultured bacterium 

The incidence of intestinal diseases increases with age, but the regulators of gut aging and the molecules linking gut aging and diseases remain largely unknown. By profiling the transcriptome, proteome, phosphoproteome and metabolome of 104 Macaca fascicularis large intestinal tissues, ...

2023-12-14 | MTBLS7612 | MetaboLights
For proteomic analysis, total protein was extracted from 3000 oocytes of each genotype and then analyzed by PTM-biolab (China).
ORGANISM(S): Mus Musculus 
Exercise, as the fundamental therapy for metabolic diseases, often exhibits a dose-dependent fashion. However, the adverse effects of overtraining and underlying mechanisms remain unclear. Here, we show that long-term overtraining induces hepatic fibrosis in humans and mice. Mechanistically, excessi...
ORGANISM(S): Mus Musculus 
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