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Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
ProteomicsMirror-image trypsin digestion and sequencing of D-proteins
The development of mirror-image biology systems and related applications is hindered by the lack of effective methods to sequence mirror-image (D-) proteins. Although natural-chirality (L-) proteins can be sequenced by bottom–up liquid chromatography–tandem mass spectrometry (LC–MS/MS), the sequenci...
ORGANISM(S): Escherichia coli 
2024-01-19 | PXD046228 | Pride
Total chemical synthesis D-trypsin D-protein sequencing Mirror-image biology Lc-ms/ms 
ProteomicsComparative proteomic analyses of the liver in D-galactosamine sensitized mice treated with different toll-like receptor agonists
Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury with lethal outcomes. Three toll-like receptor (TLR) agonists, including polyinosinic-polycytidylic acid [poly(I:C)], lipopolysaccharide (LPS), and cytosine-phosphate-guanine oligonucleotide (CpG ODN), respectively, cause ...
ORGANISM(S): Mus Musculus 
2020-01-19 | PXD017180 |
TranscriptomicsGenome-wide expression profiling of the response to D-limonene in Saccharomyces cerevisiae
Metabolic engineering of Saccharomyces cerevisiae for efficient monoterpenes production was mostly restricted by the limited tolerance to these chemicals. Understanding of the molecular mechanisms underlying the tolerance of S. cerevisiae to monoterpenes was essential for the de novo biosynthesis th...
ORGANISM(S): Saccharomyces cerevisiae 
2013-12-23 | E-GEOD-34665 | biostudies-arrayexpress
TranscriptomicsRNA-seq analysis in Cornea epithelial cells (CECs), skin epithelial cells (SECs), LSCs after knocking down PAX6 (3-D shPAX6 LSCs) and SESCs transduced with PAX6(3-D PAX6+ SESCs) upon 3-D differentiation
Purpose: We find that Wnt7a-PAX6 axis determine corneal epithelial cell fate. To obtain global evidence for successful cell fate conversion, we performed gene expression profiling by RNA-seq on CECs, SECs, and LSCs after knocking down PAX6 and on SESCs transduced with PAX6 upon 3-D differentiation. ...
ORGANISM(S): Homo sapiens 
2014-07-11 | E-GEOD-54322 | biostudies-arrayexpress
ProteomicsQuantitative analysis to the subcellular proteome in response to cathepsin D knockdown in HeLa cells
Cathepsin D is reportedly to be closely associated with tumor development, migration and invasion, but its pathological mechanism is not fully elucidated. We aimed to evaluate phenotypic changes and molecular events in response to cathepsin D knockdown. Lowering endogenous cathepsin D abundance (CR)...
ORGANISM(S): Homo sapiens (Human) 
2015-12-17 | PXD002844 | Pride
Cytosol Lysosome Silac Nucleus Subcellular fractionation 
ProteomicsProteomic analysis of cucumber (Cucumis sativus) fruit peel in response to pre-storage cold acclimation
To distinguish PsCA-responsive proteins, total proteins extracted from the non-acclimated fruit, the fruit exposed to 3 d of cold acclimation, the control fruit stored at 5oC for 12 d and the fruit exposed to 3d of PsCA treatment followed by 9 d of cold storage were separated by 2-DE, respectively....
ORGANISM(S): Cucumis sativus 
2018-10-22 | PXD008551 | Pride
Lc-msms Cucumber fruit Cold storage Defense response Chilling stress 
UnknownThe Zhu-Lu formula: a machine learning-based intraocular lens power calculation formula for highly myopic eyes.
Not available
S-EPMC10233923 | biostudies-literature
MetabolomicsPlasma metabolite changes in dairy cows during parturition identified using untargeted metabolomics
The metabolic responses of cows undergo substantial changes during the transition from late pregnancy to early lactation. However, the molecular mechanisms associated with these changes in physiological metabolism have not been clearly elucidated. The objective of this study was to investigate metab...
2019-08-20 | MTBLS809 | MetaboLights
UnknownVitamin D-Binding Protein, Bioavailable, and Free 25(OH)D, and Mortality: A Systematic Review and Meta-Analysis.
Not available
S-EPMC9571577 | biostudies-literature
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