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ProteomicsThe MbovP280 of mycoplasma bovis immunoprecipitates with the wole cell lysates of bovine macrophage (BoMac),and then LC-MS/MS was used to identify the MbovP280-binding ligands
An IP–MS method was used to screen for MbovP280-binding proteins. Briefly, BoMac cells were cultured, harvested, and lysed in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). The whole-cell lysates (400 μg) were incubated with 10 μg of either rMbovP280 o...
ORGANISM(S): Bos taurus (Bovine) 
2021-09-09 | PXD022080 | Pride
Lc-ms/ms Bomac Bovine Ip Mycoplasma bovis Mbovp280 
TranscriptomicsMediator Med23-deficiency Enhances Neural Differentiation of Embryonic Stem Cells through Modulating BMP Signaling
Unraveling the mechanisms underlying early neural differentiation of ESCs is crucial to the cell-based therapies of neurodegenerate diseases. Neural fate acquisition is proposed to be controlled by a “default” mechanism, for which the molecular regulation is not well understood. In this study, w...
ORGANISM(S): Mus musculus 
2015-03-05 | E-GEOD-64500 | biostudies-arrayexpress
ProteomicsThe NAE1-mediated Neddylation operates as an essential post-translational modification checkpoint for effector CD8+ T cells
Cells were lysed with TFA, and precipitated with cold acetone. The precipitates were transferred to E3filters and digestion was performed overnight with trypsin. The peptides were desalted with StageTips, and were analyzed on Eclipse Orbitrap with DIA mode. For MS1, the resolution was set to 120,00...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-02-27 | MSV000094179 | MassIVE
MetabolomicsAnalysis of fecal microbiome and metabolome changes in goats with pregnant toxemia

Pregnancy toxemia is a common disease, which occurs in older ewes that are pregnant with multiple lambs in the third trimester. Most of the sick goats die within a few days, which can seriously impact the economic benefits of goat breeding enterprises. The disease is believed to be caused by maln...

2024-10-01 | MTBLS7519 | MetaboLights
UnknownIsolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China.
Not available
S-EPMC3265569 | biostudies-literature
UnknownBran-New Four-Molecule and Five-Molecule Cascade Reactions for One-Pot Synthesis of Pyrano[3,2-c]chromen-5-ones and Spiro[benzo[b][1,4]diazepine-2,2'-pyrano[3,2-c]chromen]-5'-ones under Catalyst- and Solvent-Free Conditions.
Not available
S-EPMC6217645 | biostudies-literature
TranscriptomicsNext Generation Sequencing Facilitates Comparisons of Control and Schizophrenia-Patient derived hiPSC-derived NPCs
Cell-based models of many neurological and psychiatric diseases, established by reprogramming patient somatic cells into human induced pluripotent stem cells (hiPSCs), have now been reported. While numerous reports have demonstrated that neuronal cells differentiated from hiPSCs are electrophysiolo...
ORGANISM(S): Homo sapiens 
2016-03-14 | E-GEOD-63738 | biostudies-arrayexpress
UnknownHomogenate-Ultrasound-Assisted Ionic Liquid Extraction of Total Flavonoids from Selaginella involven: Process Optimization, Composition Identification, and Antioxidant Activity.
Not available
S-EPMC8190928 | biostudies-literature
UnknownAssociations of immunological features with COVID-19 severity: a systematic review and meta-analysis.
Not available
S-EPMC8329624 | biostudies-literature
UnknownDevelopment of single nucleotide polymorphisms in key genes of taurine and betaine metabolism in Crassostrea hongkongensis and their association with content-related traits.
Not available
S-EPMC11849188 | biostudies-literature
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