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Single-cell proteomic data from RO-3306 treated alveolar type II epithelial cells (C10) and untreated C10 cells. Cells were sorted using cellenONE onto several nanoSPLITS chips. Cell lysates were then split for parallel RNAseq and proteomic analysis. For proteomics recovery experiment where both chi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-12-02 | MSV000090828 | MassIVE
We developed an ultrasensitive top down platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples), into a top down workflow. We determined that a unique combination of a nonionic detergent DDM with urea as a protein extr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-22 | MSV000084612 | MassIVE
We developed a hanging drop (HD) method to improve the sample recovery for spatial proteomics by placing the nanowell chip in an upside-down direction during droplet-based sample preparation. Compared with the commonly-used sitting-drop (SD) method, the HD method can keep the tissue voxel away from ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-04-21 | MSV000089295 | MassIVE
Rat neonatal cardiac myocytes were treated with PDE5 and PDE9 inhibitor in the presence and absence of L-NAME.
ORGANISM(S): Rattus norvegicus (Rat) 
2016-06-24 | PXD001585 | Pride
Human pancreatic islets from two human donors (Prodo Laboratories) were acquired and cultured for 24 hr in pancreatic islet media (PIMS, Prodo Laboratories). Single cells were dissociated with TrypLE for ~15-30 minutes, washed with PBS, stained with Calcein-AM/propidium iodide, and sorted onto nanoS...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-07 | MSV000093330 | MassIVE
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