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TranscriptomicsMicroarray mRNA expression profiling of E16.5 foetal gonocytes
Gonocytes were FACS sorted from testes of E16.5 Miwi2 Tom/+ embryos using the tdTomato reporter knocked into the Miwi2 locus. RNA was isolated from sorted cells and transcript expression analyzed by microarray measurement.
ORGANISM(S): Mus musculus 
2020-06-18 | E-MTAB-7985 | biostudies-arrayexpress
GenomicsSPOCD1 is a novel executor of piRNA-directed DNA methylation that links MIWI2 to the de novo DNA methylation machinery
In mammals, the acquisition of the germline from the soma provides the germline with an essential challenge, the necessity to erase and reset genomic methylation. In the male germline RNA-directed DNA methylation silences young active transposable elements (TEs). The PIWI protein MIWI2 (PIWIL4) and ...
ORGANISM(S): Mus musculus 
2020-06-01 | E-MTAB-7997 | biostudies-arrayexpress
GenomicsC19ORF84 connects piRNA and DNA methylation machineries to defend the mammalian germline
In the male mouse germline, PIWI-interacting RNAs (piRNAs), bound by the PIWI protein MIWI2 (PIWIL4), guide the DNA methylation of young active transposable elements (TEs) through SPOCD1. We identified loss-of-function variants in human SPOCD1 that cause male infertility and defective TE silencing. ...
ORGANISM(S): Mus musculus 
2024-02-13 | E-MTAB-11612 | biostudies-arrayexpress
GenomicsDNA methylation of yound male wild type and dSpin1 knockout mice
In the male mouse germline, PIWI-interacting RNAs (piRNAs), bound by the PIWI protein MIWI2 (PIWIL4), guide the DNA methylation of young active transposable elements (TEs) through SPOCD1.
ORGANISM(S): Mus musculus 
2024-07-22 | E-MTAB-12713 | biostudies-arrayexpress
ProteomicsTwo-factor authentication underpins the precision of piRNA-directed LINE1 DNA methylation
The PIWI-interacting RNA (piRNA) pathway guides the DNA methylation of young active transposons during male mouse germline development. piRNAs tether the PIWI protein MIWI2 (PIWIL4) to the nascent transposon transcript that results in DNA methylation through SPOCD1. Transposon methylation requires e...
ORGANISM(S): Mus musculus (Mouse) 
2024-09-04 | PXD041214 | Pride
Transposon Pirna Germline Dna methylation Piwi 
ProteomicsC19ORF84 connects piRNA and DNA methylation machineries to defend the mammalian germline
In the male mouse germline, PIWI-interacting RNAs (piRNAs), bound by the PIWI protein MIWI2 (PIWIL4), guide DNA methylation of young active transposons through SPOCD1. However, the underlying mechanisms of SPOCD1-mediated piRNA-directed transposon methylation and whether this pathway functions to pr...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-14 | PXD047331 | Pride
C19orf84 Spocd1 Pirna Dna methylation 
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