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ProteomicsDetection, isolation and characterization of phage-host complexes using BONCAT and click chemistry
Phages are viruses that infect prokaryotes and can shape microbial communitiesby lysis, thus offering applications in various fields. However, challengesexist in sampling, isolation and accurate prediction of the host specificity ofphages as well as in the identification of newly replicated virions ...
ORGANISM(S): Escherichia phage Lambda 
2024-08-29 | PXD044316 | Pride
Phage Fluorescence Proteomics Boncat labeling Host range Sequencing Click chemistry Biotin Lc-ms/ms 
UnknownA Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using Phenol Extraction, FASP Digest, and the MetaProteomeAnalyzer.
Not available
S-EPMC6707425 | biostudies-literature
UnknownMetaproteome analysis reveals that syntrophy, competition, and phage-host interaction shape microbial communities in biogas plants.
Not available
S-EPMC6486700 | biostudies-literature
ProteomicsBiogas Plant Survey Magdeburg 2014-2016
Biogas plants (BGPs) produce methane and carbon dioxide through the anaerobic digestion of agricultural waste. Identification of strategies for more stable biogas plant operation and increased biogas yields require better knowledge about the individual degradation steps and the interactions within t...
ORGANISM(S): environmental samples <Methanosarcinales> 
2019-05-03 | PXD009349 | Pride
Anaerobic digestion Biogas process Competition Bacteriophages Anaerobic digestion model 1 Microbial communities Metaproteomeanalyzer Host-phage interactions Syntrophy Metaproteomics 
ProteomicsA robust and universal metaproteomics workflow for research studies and routine diagnostics within 24 hours
A new workflow for metaproteomics is presented in this study and is shown to enable the analysis of a broad range of different samples in only 24 h. The standardized sample preparation includes the combined cell lysis and phenol extraction in a ball mill followed by FASP digestion. The bioinformatic...
ORGANISM(S): Enterobacteriaceae sp. Methanosarcinales 
2019-09-04 | PXD010550 | Pride
Microbial communities Mass spectrometry Environmental proteomics Software Bioinformatics Sample preparation 
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