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Mediator complex regulates transcription by connecting enhancers to promoters. High Mediator binding density defines super enhancers, which regulate cell-identity genes and oncogenes. Protein interactions of Mediator may explain its role in these processes but have not been identified comprehensivel...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-06 | PXD013546 | Pride
DNA-protein crosslinks (DPCs), arise from enzymatic intermediates, endogenous metabolism or exogenous chemicals like chemotherapeutics. DPCs are highly cytotoxic as they will impede DNA transacting processes such as replication, which is counteracted by proteolysis-mediated DPC removal by the protea...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2024-02-09 | PXD041679 | Pride
Mouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-11-30 | MSV000083176 | MassIVE
Human monocyte-derived DC were cultured at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 10% FCS, 1% Pen/Strep, 10 ng/ml GM-CSF and 10 ng/ml IL4. At day 7, they were stimulated or not with 500 ng/ml LPS. Cells were collected at day 10. Whole cell lystaes from 2x10E8 cells were used f...
ORGANISM(S): Homo Sapiens 
2018-11-30 | MSV000083175 | MassIVE
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