Comparative genomic hybridization of human NA, NP1, NR1, NR15 cells denoting different re-activiations of EBV to investigate the effect of highly recurrent EBV reactivation on genetic copy number alterations

ABSTRACT: We proposed that the frequency of EBV reactivation may be crucial for its pathogenic role and highly recurrent EBV reactivations exert a profound influence on genome instability. Recurrent reactivations were induced in the EBV-latently infected NPC cells (NA cells) by treating with 12-o-tetradecanoylphorbol-13-acetate (TPA) and sodium n-butyrate (SB) once per passage periodically. Genome-wild oligoarray-based comparative genomic hybridization (CGH) technology was applied to investigate the copy-number aberrations in response for different frequencies of EBV reactivation. The results showed that the NR15 cells, NA cells with the highest frequency (15 times) of reactivation, exhibit extensive genomic copy-number alterations (CNAs) mostly involving chromosome 3p, 3q, 8p, 8q, 9p and 9q, wherea

ORGANISM(S): Homo sapiens

SUBMITTER: Chia Huei Lee 

PROVIDER: E-GEOD-6472 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress