Transcription profiling of rat esophagus treated with N-nitrosomethylbenzylamine to identify the effects of phenylethyl isothiocyanate on N-nitrosomethylbenzylamine-induced cytotoxicity

ABSTRACT: To date, there is little information on early molecular events in the development of N-nitrosomethylbenzylamine (NMBA) induced esophageal tumorigenesis and of the effects of chemopreventive agents on these events. In this study, we identified genes in rat esophagus that were differentially expressed in response to shortâ??term NMBA-treatment and modulated by co-treatment with phenethylisothiocyanate (PEITC). Rats were fed AIN-76A diet or AIN-76A diet containing PEITC for three weeks. During the third week of dietary treatment, they were administered three s.c. doses of NMBA (0.5 mg/kg b.w.). Rats were sacrificed 24 h after the last treatment, esophagi excised and processed for histological grading, microarray and real-time PCR analysis. Our histopathological data showed that treatment of r

ORGANISM(S): Rattus norvegicus

SUBMITTER: Gary Stoner 

PROVIDER: E-GEOD-6917 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress