Endosialin-positive tumor-derived pericytes promote tumor progression through impeding the infiltration of CD8⁺ T cells in clear cell renal cell carcinoma.

ABSTRACT:

Background

Immune checkpoint blockade (ICB) therapy can be effective against clear cell renal cell carcinoma (ccRCC), but many patients show no benefit. Tumor-derived pericytes (TDPs) may promote tumor progression by influencing T cells and are an immunotherapy target; however, they may comprise functionally distinct subtypes. We aimed to identify markers of tumor-promoting TDPs and develop TDP-targeting strategies to enhance ICB therapy effectiveness against ccRCC.

Methods

We analyzed the relationship between endosialin (EN) expression and cytotoxic T-lymphocyte (CTL) infiltration in ccRCC tumor samples using flow cytometry and in a ccRCC-bearing mice inhibited for EN via knockout or antibody-mediated blockade. The function of EN^high TDPs in CTL infiltration and

SUBMITTER: Lu T 

PROVIDER: S-EPMC10198862 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature