Statistical optimization of P(3HB-co-3HHx) copolymers production by Cupriavidus necator PHB^-4/pBBR_CnPro-phaC_Rp and its properties characterization.

ABSTRACT: Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] is a bacterial copolymer in the polyhydroxyalkanoates (PHAs) family, a next-generation bioplastic. Our research team recently engineered a newly P(3HB-co-3HHx)-producing bacterial strain, Cupriavidus necator PHB^-4/pBBR_CnPro-phaC_Rp. This strain can produce P(3HB-co-2 mol% 3HHx) using crude palm kernel oil (CPKO) as a sole carbon substrate. However, the improvement of P(3HB-co-3HHx) copolymer production by this strain has not been studied so far. Thus, this study aims to enhance the production of P(3HB-co-3HHx) copolymers containing higher 3HHx monomer compositions using response surface methodology (RSM). Three significant factors for P(3HB-co-3HHx) copolymers production, i.e., CPKO concentration, sodium h

SUBMITTER: Trakunjae C 

PROVIDER: S-EPMC10237529 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature