Themis controls T cell activation, effector functions, and metabolism of peripheral CD8⁺ T cells.

ABSTRACT: Themis is important in regulating positive selection of thymocytes during T cell development, but its role in peripheral T cells is less understood. Here, we investigated T cell activation and its sequelae using a tamoxifen-mediated, acute Themis deletion mouse model. We find that proliferation, effector functions including anti-tumor killing, and up-regulation of energy metabolism are severely compromised. This study reveals the phenomenon of peripheral adaptation to loss of Themis, by demonstrating direct TCR-induced defects after acute deletion of Themis that were not evident in peripheral T cells chronically deprived of Themis in dLck-Cre deletion model. Peripheral adaptation to long-term loss was compared using chronic versus acute tamoxifen-mediated deletion and with the (chronic) dL

SUBMITTER: Gautam N 

PROVIDER: S-EPMC10517225 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature