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Ratiometric fluorescence sensing and quantification of circulating blood sodium sensors in mice in vivo.

ABSTRACT: In this work, we introduce ratiometric diffuse in vivo flow cytometry (R-DiFC) for quantitative measurement of circulating fluorescent red blood cell (fRBC) sensors for systemic blood sodium levels. Unlike in our previous work in measuring circulating fRBC sensors, R-DiFC allows simultaneous measurement of two fluorophores encapsulated in the sensor, the ratio of which enables self-calibration of the fluorescence signal with different fRBC depths in biological tissue. We show that the R-DiFC signal varies significantly less than either fluorescence signal alone. This work holds promise for personalized monitoring of systemic sodium for bipolar patients in the future.

SUBMITTER: Ivich F

PROVIDER: S-EPMC10659809 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Ratiometric fluorescence sensing and quantification of circulating blood sodium sensors in mice in vivo.

Ivich Fernando F Calderon Isen I Fang Qianqian Q Clark Heather H Niedre Mark M

Biomedical optics express 20231003 11

In this work, we introduce ratiometric diffuse in vivo flow cytometry (R-DiFC) for quantitative measurement of circulating fluorescent red blood cell (fRBC) sensors for systemic blood sodium levels. Unlike in our previous work in measuring circulating fRBC sensors, R-DiFC allows simultaneous measurement of two fluorophores encapsulated in the sensor, the ratio of which enables self-calibration of the fluorescence signal with different fRBC depths in biological tissue. We show that the R-D ...[more]

PMID: 38021147

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