Spatial distribution pattern of immune cells is associated with patient prognosis in colorectal cancer.

ABSTRACT:

Background

The spatial context of tumor-infiltrating immune cells (TIICs) is important in predicting colorectal cancer (CRC) patients' clinical outcomes. However, the prognostic value of the TIIC spatial distribution is unknown. Thus, we aimed to investigate the association between TIICs in situ and patient prognosis in a large CRC sample.

Methods

We implemented multiplex immunohistochemistry staining technology in 190 CRC samples to quantify 14 TIIC subgroups in situ. To delineate the spatial relationship of TIICs to tumor cells, tissue slides were segmented into tumor cell and microenvironment compartments based on image recognition technology, and the distance between immune and tumor cells was calculated by implementing the computational pipeline phenoptr.

Results

SUBMITTER: Shen R 

PROVIDER: S-EPMC11218284 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature