Self-Organization of Sinusoidal Vessels in Pluripotent Stem Cell-derived Human Liver Bud Organoids.

ABSTRACT: The induction of tissue-specific vessels in in vitro living tissue systems remains challenging. Here, we directly differentiated human pluripotent stem cells into CD32b⁺ putative liver sinusoidal progenitors (iLSEP) by dictating developmental pathways. By devising an inverted multilayered air-liquid interface (IMALI) culture, hepatic endoderm, septum mesenchyme, arterial and sinusoidal quadruple progenitors self-organized to generate and sustain hepatocyte-like cells neighbored by divergent endothelial subsets composed of CD32b^lowCD31^high, LYVE1⁺STAB1⁺CD32b^highCD31^lowTHBD^-vWF^-, and LYVE1^-THBD⁺vWF⁺ cells. Wnt2 mediated sinusoidal-to-hepatic intercellular

SUBMITTER: Saiki N 

PROVIDER: S-EPMC11245015 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature