Single nucleotide polymorphism profiles of canine T-cell and null-cell lymphomas.

ABSTRACT:

Background

The histopathological classification of T-cell lymphoma (TCL) in humans has distinctive mutational genotyping that suggests different lymphomagenesis. A similar concept is assumed to be observed in dogs with different TCL phenotypes.

Objective

This study aimed to identify the previously reported single-nucleotide polymorphisms (SNPs) in both human beings and dogs in canine TCLs and null-cell lymphomas (NCLs) and to design compatible oligonucleotides from each variant based on the multiplex polymerase chain reaction.

Methods

Genomic DNA was extracted from 68 tumor specimens (62 TCLs and 6 NCLs) and 5 buffy coat samples from dogs with TCL. Four TCL subtypes and NCL were analyzed in 44 SNPs from 21 genes using the MassARRAY.

Results

The greatest inciden

SUBMITTER: Sirivisoot S 

PROVIDER: S-EPMC11339873 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature