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Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing.

ABSTRACT: Transduction of producer cells during lentiviral vector (LVV) production causes the loss of 70-90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the LDL receptor located on the producer cell membrane, allowing lentiviral vector transduction. Avoiding retro-transduction in LVV manufacturing is crucial to improve net production and, therefore, the efficiency of the production process. Here, we describe a method for quantifying the transduction of producer cells and three different strategies that, focused on the interaction between VSV-G and the LDLR, aim to reduce retro-transduction.

SUBMITTER: Banos-Mateos S

PROVIDER: S-EPMC11359676 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing.

Banos-Mateos Soledad S Lopez-Robles Carlos C Yubero María Eugenia ME Jurado Aroa A Arbelaiz-Sarasola Ane A Lamsfus-Calle Andrés A Arrasate Ane A Albo Carmen C Ramírez Juan Carlos JC Fertin Marie J MJ

Viruses 20240729 8

Transduction of producer cells during lentiviral vector (LVV) production causes the loss of 70-90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the LDL receptor located on the producer cell membrane, allowing lentiviral vector transduction. Avoiding retro-transduction in LVV manufacturing is crucial to improve net production and, therefore, the efficiency of the production process. Here, we ...[more]

PMID: 39205190

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