Sensitive SERS Platform for the Detection of via Freezing SERS Tags and Core-Shell Aptamer-Au@Fe₃O₄ Nanoparticles.

ABSTRACT: The detection of is crucial for the diagnosis and treatment of infectious diseases. However, developing a simple and sensitive method for detection remains challenging. Herein, we propose a sensitive surface-enhanced Raman spectroscopy (SERS) platform for the detection of using core-shell aptamer-Au@Fe₃O₄ nanoparticles (Fe₃O₄-Au@apt) and freezing SERS tags. Core-shell Au@Fe₃O₄ magnetic nanoparticles were modified with specific primary aptamers for precise and efficient capture of , followed by modification with large amounts of secondary aptamers and tetramercaptobenzoic acid through freezing. The proposed SERS platform demonstrated significant sensitivity toward , with a limit of detection of 6.91 CFU/mL, which demonstrates 99.25 times higher sensitivity than conventional SERS tags. Additionally, the proposed SERS platform achieved good recovery rates in human urine and serum samples, ranging from 97.7% to 104.0% and 90.5% to 104.7%. The findings of the study suggest that the proposed SERS platform holds promise for the rapid and efficient detection of pathogenic bacteria.

SUBMITTER: Zheng M 

PROVIDER: S-EPMC12290702 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature