Computational discovery of sense-antisense transcription in the human and mouse genomes.

ABSTRACT:

Background

Overlapping but oppositely oriented transcripts have the potential to form sense-antisense perfect double-stranded (ds) RNA duplexes. Over recent years, the number and variety of examples of mammalian gene-regulatory phenomena in which endogenous dsRNA duplexes have been proposed or demonstrated to participate has greatly increased. These include genomic imprinting, RNA interference, translational regulation, alternative splicing, X-inactivation and RNA editing. We computationally mined public mouse and human expressed sequence tag (EST) databases to search for additional examples of bidirectionally transcribed genomic regions.

Results

Our bioinformatics approach identified over 217 candidate overlapping transcriptional units, almost all of which are novel. From e

SUBMITTER: Shendure J 

PROVIDER: S-EPMC126869 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature