Transplantation of adult monkey neural stem cells into a contusion spinal cord injury model in rhesus macaque monkeys.

ABSTRACT:

Objective

Currently, cellular transplantation for spinal cord injuries (SCI) is the subject of numerous preclinical studies. Among the many cell types in the adult brain, there is a unique subpopulation of neural stem cells (NSC) that can self-renew and differentiate into neurons. The study aims, therefore, to explore the efficacy of adult monkey NSC (mNSC) in a primate SCI model.

Materials and methods

In this experimental study, isolated mNSCs were analyzed by flow cytometry, immunocytochemistry, and RT-PCR. Next, BrdU-labeled cells were transplanted into a SCI model. The SCI animal model was confirmed by magnetic resonance imaging (MRI) and histological analysis. Animals were clinically observed for 6 months.

Results

Analysis confirmed homing of mNSCs into the injur

SUBMITTER: Nemati SN 

PROVIDER: S-EPMC4072074 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature